中国病理生理杂志2011，Vol.27Issue(9)：1665-1670,6.DOI:10.3969/j.issn.1000-4718.2011.09.001
压力负荷诱导大鼠心肌重塑早期左室舒张功能评价
Evaluation of left ventricular diastolic dysfunction at the early stage of pressure overload-induced myocardial remodeling in rats
摘要
Abstract
AIM: To investigate the evaluation method of left ventricular diastolic function and myocardial hypertrophy induced by pressure overload in rats.METHODS: Male Sprague - Dawley rats were subject to left ventricular pressure overload by transverse aortic constriction ( TAC ).Cardiac structure and diastolic function were evaluated by echocardiography, hemodynamic analysis and examination of hydroxyproline concentration in the myocardial tissues.RESULTS : Compared with the sham - operated controls, left ventricular wall dimension in diastole significantly increased in the rats 3 weeks after TAC [anterior wall: ( 1.71 ±0.11 ) mm vs ( 1.43 ±0.06 ) mm, P ＜0.01; posterior wall:( 1.78 ± 0.03 ) mm vs ( 1.44 ±0.02 ) mm, P ＜0.01].Left ventricular early diastolic posterior wall motion velocity ( E' ) significantly decreased in the rats 3 weeks after TAC[( 52.1 ±0.8 ) mm · s-1 vs ( 63.8 ± 1.5 ) mm · s-1 , P ＜0.01], and was much lower than that in the rats 6 weeks after TAC.Left ventricular mass to tibia length in TAC rats was much higher than that in sham - operated controls [3 weeks after TAC: ( 19.6 ± 1.1 ) mg · mm-1 vs ( 14.8 ± 0.6 ) mg · mm-1 , P ＜ 0.01; 6 weeks after TAC: ( 23.2 ±0.9 ) mg · mm-1 vs ( 15.6 ±0.7 ) mg · mm-1 , P ＜0.01].The ratio of maximum rate of degression of left ventricular pressure ( dp/dtmin ) to left ventricular systolic pressure ( dp/dtmin/LVSP ) started to decrease in TAC rats in the 3rd week ( 48.9 ± 5.9 vs 63.5 ± 9.9 ) and significantly decreased in TAC rats in the 6th week as compared with sham - operated controls ( 35.4 ±4.0 vs 54.4 ± 2.9, P ＜0.01 ).Sirius red - stained collagen in cardiac interstitium, especially around the blood vessels, was increased in TAC rats.Six weeks after TAC, a significant increase in the content of myocardial hydroxyproline[( 465.3 ± 17.3 ) μg vs ( 351.4 ±8.2 ) μg, P ＜0.05]was observed.CONCLUSION: The early diastolic posterior wall motion velocity ( E' ) detected by tissue Doppler imaging is a sensitive indicator of diastolic dysfunction at the early stage of myocardial remodeling induced by pressure overload in rats.关键词
压力负荷/大鼠/舒张功能/心肌重塑Key words
Pressure overload/ Rats/ Diastolic function/ Myocardial remodeling分类
医药卫生引用本文复制引用
盖迎利,徐明,白燕,肖晗,马晓伟,吕志珍,冯杰莉,张幼怡,李昭屏..压力负荷诱导大鼠心肌重塑早期左室舒张功能评价[J].中国病理生理杂志,2011,27(9):1665-1670,6.基金项目
国家自然科学基金资助项目(No.81072635) （No.81072635）
国家自然科学基金重点资助项目(No.81030001) （No.81030001）
国家自然科学基金国际合作与交流资助项目(No.30910103902) （No.30910103902）
