中国病理生理杂志2011，Vol.27Issue(9)：1671-1675,5.DOI:10.3969/j.issn.1000-4718.2011.09.002
肝X受体抑制乳鼠心肌细胞缺氧/复氧损伤
Liver X receptors attenuate anoxia/reoxygenation injury in neonatal rat cardiomyocytes
摘要
Abstract
AIM: To investigate the effect of liver X receptors ( LXRs ) on anoxia/reoxygenation injury in cultured neonatal rat cardiomyocytes and to explore the underlying cellular signaling pathway.METHODS: Neonatal rat cardiomyocytes were cultured and treated with anoxia/reoxygenation in the absence or presence of T0901317 ( an agonist of LXRs ) at various doses (1,5 and 10 μmol/L ).LDH and CK were detected by colorimetry 24 h after reoxygenation.The relative transcription levels of proinflammatory factors, such as tumor necrosis factor α( TNF - α ), interleukin - 6( IL - 6 ) and monocyte chemoattractant protein - l( MCP - 1 ),were quantified by real - time PCR.H9c2 cells were infected with adenoviral vectors containing constitutively - active forms of LXRα and LXRβ, then underwent anoxia/reoxygenation procedure.The activity of NF - κB in the cells was detected by luciferase assay.RESULTS: Treatment with T0901317 significantly suppressed anoxia/reoxygenation - induced increases in LDH and CK ( P ＜0.05 ).Transcription levels of proinflammatory factors in T0901317 treatment groups were significantly lower than those in control group ( P ＜0.05 ).Adenovirus -mediated overexpression of constitutively - active LXRs suppressed anoxia/reoxygenation - induced NF - κB activity in H9c2 cells.CONCLUSION: LXRs are negative regulators of myocardial ischemia - reperfusion injury.关键词
X受体,肝/心肌缺血/再灌注损伤/细胞培养Key words
X receptors,liver/ Myocardial ischemia - reperfusion injury/ Cell culture分类
医药卫生引用本文复制引用
殷然,王梦洪,郑泽琪,彭锦添,夏莺..肝X受体抑制乳鼠心肌细胞缺氧/复氧损伤[J].中国病理生理杂志,2011,27(9):1671-1675,5.基金项目
国家自然科学基金资助项目(No.81000071) （No.81000071）
