中国病理生理杂志2011，Vol.27Issue(9)：1802-1806,5.DOI:10.3969/j.issn.1000-4718.2011.09.027
病理性瘢痕与正常皮肤的蛋白质组学研究
Proteomic analysis between pathological scars and normal skin
摘要
Abstract
AIM: To investigate and screen the sensitive proteins in the formation mechanism of pathological scars by comparing the results of differential proteomic analysis between pathological scars and normal skin.METHODS: Two - dimensional gel electrophoresis was used to detect the protein expression profiles in 8 keloid patients, 8 hypertrophic scar patients and 3 matched normal skin patients.The proteins that showed differential expression of over 4 - fold change were cut and analyzed by MALDI - TOF/TOF mass spectrometry.RESULTS: A two - dimensional protein profiling comparison between pathological scars and normal skin was successfully established.On average, 2 978 spots in keloid, 2 975 spots in hypertrophic scar and 3 053 spots in normal skin were identified using gel analysis software.Compared with normal skin, there were totally 36 differentially - expressed proteins in keloid and hypertrophic scar identified from the spots of over 4 - fold change, including 16 proteins in both keloid and hypertrophic scar ( 8 up - regulated and 8 down - regulated ), 11 only in keloid ( 9 up - regulated and 2 down - regulated ) and 9 only in hypertrophic scar ( 4 up - regulated and 5 down - regulated ).CONCLUSION: Proteomic analysis can identify the proteins with variance of pathological scars versus normal skin, thus providing probable new clues to reveal the formation mechanism of pathological scars.关键词
瘢痕疙瘩/瘢痕,增生性/蛋白质组学/波形蛋白/Asporin/OsteoglycinKey words
Keloid/ Scars,hypertrophic/ Proteomics/ Vimentin/ Asporin/ Osteoglycin分类
医药卫生引用本文复制引用
胡志成,朱家源,朱斌,郭栋,陈斌,张凯,胡坤华,黎明涛,唐冰..病理性瘢痕与正常皮肤的蛋白质组学研究[J].中国病理生理杂志,2011,27(9):1802-1806,5.基金项目
国家自然科学基金资助项目(No.30973128) （No.30973128）
广东省自然科学基金资助项目(No.8151008901000082) （No.8151008901000082）
广东省自然科学基金资助项目(No.2007B031504003) （No.2007B031504003）
