医药导报2011，Vol.30Issue(9)：1147-1150,4.DOI:10.3870/yydb.2011.09.008
染料木素对人宫颈癌Siha细胞增殖凋亡和周期的影响
Effects of Genistein on Proliferation,Apoptosis and Cell Cycle of Human Cervical Cancer Cell Line Siha
摘要
Abstract
Objective To investigate the effect of genistein on cell proliferation, apoptosis and cell cycle of human cervical cancer cell line Siha. Methods Siha cells were incubated with 50 mol· mL-1 genistein diluted in DMSO or DMSO at equal volume. The cell growth was measured by MTT. Cell apoptosis, cell cycle and expression of cyclin A and cyclin B were detected by means of flow cytometry( PCM). Results The proliferation of Siha cells treated with genistein was significantly inhibited compared to the control group (P＜0. 05) ; PCM assay showed that cell apoptosis was increased by being treated with genistein for 48 h(67.45±l. 50)％ compared to that in the control(45. 37±1. 65)％ (P＜0.01) ; cells arrested at G2/M phase were increased from(9. 55±0.71)％ to(79. 54±1.09)％ (P＜0.01); the protein expression of cyclin A and cyclin B were up-regulated from(l. 84 ±0.11)％ to(37.38±0.71)％ (P＜0.01) and from(69.44±1.84)％ to(97.70±1.20)％ (P＜0.01), respectively Conclusion Genistein inhibits the growth of cervical cancer cell line Siha in vitro, and the mechanisn of which is likely due to up-regulation of cyclin A and cyclin B and arresting cell cycle at G2/M phase.关键词
染料木素/宫颈癌细胞/细胞增殖/细胞凋亡/细胞周期Key words
Genistein/ Cervical cancer cell/ Cell proliferation /Cell apoptosis/ Cell cycle分类
医药卫生引用本文复制引用
李莉,王薇,廖书杰,李伟,刘眈,曹波,李科珍..染料木素对人宫颈癌Siha细胞增殖凋亡和周期的影响[J].医药导报,2011,30(9):1147-1150,4.基金项目
国家自然科学基金资助项目(基金编号:81072132,30901586,81001152) （基金编号:81072132,30901586,81001152）
