中国组织工程研究与临床康复2011，Vol.15Issue(27)：5035-5038,4.DOI:10.3969/j.issn.1673-8225.2011.27.021
同种异基因成骨细胞对混合淋巴细胞反应体系中T细胞的免疫抑制
Immunosuppression of allogeneic bone marrow-derived mesenchymal stem cells-induced osteoblasts on T cells in mixed lymphocyte culture system
摘要
Abstract
BACKGROUND: Results of immunclogical response of allogeneic bone marrow-derived mesenchymal stem cells-induced osteoblast transplantation are varied.OBJECTIVE: To investigate the features of immune adjustment function derived from the interaction of osteoblasts derived from mesenchymal stem cells (ODCs) with T cells in vitro. METHODS: Rabbit bone marrow mononuclear cells were isolated by Ficoll-Hypaque density gradient, exvivo culture-expanded,and obtained after the third passage. The classi c chemical induced ODCs at different proportion were added to T cells to form two-way mixed lymphocyte culture system. At 3, 5, 7 days, MTT assay was used to detect T cell proliferation in each group. T -cell subsets apoptosis was analyzed using flow cytometric analysis after 24 hours.RESULTS AND CONCLUSION: ODCs inhibited T cell proliferation in a dose -effect manner in a certain range. With increasing ODC dose and time, inhibition was enhanced. Comparison of mean inhibition rate of three times showed that (1∶ 20) group was less than (1∶ 80) group, and (1∶ 40) group was less than (1∶ 80) group (P ＜ 0. 05). ODCs can cause apoptosis of T cell subsets,including CD4+ (apoptosis rate 8.57％) subtypes less than CD8+ (apoptosis 15.31％; P ＜ 0.01). Results showed that ODCs in vitro can suppress T cells proliferation through pathway of cell apoptosis, especially for CD8 +. However, this inhibition is not particularly strong, showing that the ODCs have some degree of immunity, but it is very low.关键词
混合淋巴细胞/成骨细胞/骨髓间充质干细胞/增殖/凋亡分类
医药卫生引用本文复制引用
盘荣贵,范鍥,邓耀良,赵劲民..同种异基因成骨细胞对混合淋巴细胞反应体系中T细胞的免疫抑制[J].中国组织工程研究与临床康复,2011,15(27):5035-5038,4.基金项目
医院启动项目资金资助. （）
