摘要
Abstract
BACKGROUND: The myostatin plays an important role in the regulation of muscular growth in human body, however, there is few study regarding this gene in Chinese populations.OBJECTIVE: To elucidate the association of growth differentiation factor 8 (GDF-8) gene polymorphism and human muscles through the observation of the Alu I restriction site of GDF-8 gene polymorphism.METHODS: A total of 92 undisciplined male students, of Han population, received strength training for 8 weeks at Tianjin University of Sport, three days a week. The thickness of the biceps and quadriceps was measured with the ultrasonic B test before and after exercise. The body fat, lean body weight, body weight and height were also measured before and after the exercise, as wellas the difference of Alu I restriction site of GDF-8 gene polymorphism. The length of fragments containing Alu I site was 135 bp after primer amplification, and defined as A. after Alu I restriction enzyme digestion, the allele containing Alu I site appeared fragments at 80 and 55 bp, defining as T. Thus three genotypes emerged, namely AA, AT and TT.RESULTS AND CONCLUSION: From the morphologic index, the height, weight, lean body weight, muscle thickness, the biceps and quadriceps muscle diameter of the males at Alu I site A/T heterozygote and T/T homozygote were significantly high than those at A/A homozygote (P ＜ 0.05 or 0.01). The Alu I polymorphism at GDF-8 gene loci is associated with the weight, height and lean body weight. The allele T is the congenital sensitive factor for the muscular growth.关键词
肌肉肥大/肌肉生长抑制素/力量训练/基因多态性/关联研究分类
基础医学
