摘要
Abstract
Objective To evaluate the effects of ω - 3 fat emulsion on bleomycin - induced pulmonary fibrosis by bleomycin in rats. Methods Ninety rats were randomly divided into 3 groups : NS group ( control group ), pulmonary fibrosis group ( model group ) and ω - 3 fat emulsion treatment group ( treatment group ). On 7th, 14th and 2lst day, the left lung tissues were obtained for assessment of TGF - β1 and IFN - γ expression with immunohistometry. The right lung tissues were obtained for HE staining. Meanwhile, serum IFN -γ and IL -4 were assessed with ELISA. Results ( 1 )Significantly milder pulmonary alveolitis and fibrosis were revealed in treatment group than those in model group. ( 2 )Strong positive TGF - β1 protein expression with weakly positive IFN - γ was detected on bronchiolar epithelium in model group. However, the significantly opposite expression was revealed in treatment group ( P ＜ 0. 01 ). Meanwhile, on the 7th, 14th and 21st day, significantly down - regulation and up - regulation of TGF - β1 and IFN - γ , respectively, were observed in the treatment group following time - dependent manners ( P ＜ 0. 05 ). ( 3 ) Significantly higher IL -4 and lower IFN - γ were revealed in model group than those in treatment group ( P ＜ 0. 01 ). Meanwhile, on the 7th, 14th and 21st day, significantly down - regulation and up - regulation of IL - 4 and IFN - γ, respectively, were observed in the treatment group following time - dependent manners ( P ＜ 0. 05 ). Conclusion The ω - 3 fat emulsion can alleviate pulmonary fibrosis via down - regulation of TGF - β1 protein and IL -4 levels and up - regulation of IFN - γ protein.关键词
肺纤维化/博莱霉素/γ干扰素/ω-3鱼油脂肪乳/转化生长因子β1/白细胞介素-4Key words
idiopathie pulmonary fibrosis/ bleomycin/ interferon garmma/ ω - 3 fat emulsion/ transforming growth factor - β1 / interleukin -4
