医学分子生物学杂志2011，Vol.8Issue(4)：283-288,6.DOI:10.3870/j.issn.1672-8009.2011.04.001
视黄酸核受体γ重组腺病毒构建及其鉴定
Construction and Identification of Recombinant Adenoviral Vector Carrying Rat Retinoic Acid Receptor γ Gene
摘要
Abstract
Objective To construct a recombinant adenovirus carrying rat retinoic acid receptor γ ( RARγ) gene, and research the function of RARγ in the process of differentiation of mesenchymal stem cells ( MSCs ) into neuronal-like cells. Methods Rat RARγ gene was amplified and cloned into pAdTrace-TOX vector. The recombinant vector pAd-RARγ was transfected into HEK 293 cell lines to package recombinant adenovirus Ad-RARγ. Rat MSCs were infected by Ad-RARγ endoviruses. Expression of RARγ was detected by Real-time PCR and Western blot. Results The adenovirus vector containing RARγ gene was correctly constructed, as confirmed by sequencing. Infection efficiency of Ad-RARγ in MSCs reached t0 60-70％. Both RARγ mRNA and protein expression levels in of MSCs were increased significantly by Ad-RARγ infection. Conclusion The recombinant adenovirus carrying rat RARγ gene is capable of upregulating RARγ expression in MSCs.关键词
视黄酸核受体γ/重组腺病毒/视黄酸信号转导通路/骨髓间充质干细胞Key words
retinoic acid receptor γ recombinant adenovirus/ retinoic acid signaling pathway / mesenchymal stem cells分类
生物科学引用本文复制引用
龚敏,毕杨,张赟,江伟,陈洁,魏小平,刘友学,李廷玉..视黄酸核受体γ重组腺病毒构建及其鉴定[J].医学分子生物学杂志,2011,8(4):283-288,6.基金项目
国家自然科学基金(No.30830106,No.30972461),重庆市自然科学基金(No.CSTC,2010BB5097),中国博士后基金(No.20100800667) （No.30830106,No.30972461）
