医学分子生物学杂志2011，Vol.8Issue(4)：289-293,5.DOI:10.3870/j.issn.1672-8009.2011.04.002
脂肪酸诱导胰岛β细胞凋亡过程中FoxO1对MTP的转录调控研究
Study on Transcriptional Mechanism of FoxO1 Target Gene MTP in Palmitate-induced Pancreatic β Cell Apoptosis
摘要
Abstract
Objective To study the transcriptional regulatory mechanism of MTP as FoxO1 target gene in palmitate-induced pancreatic β cells apoptosis. Methods MTT assay and Hoechst staining were used to detect the apoptosis of MIN6 cells. The mRNA level of MTP was examined using Real-time PCR assay. Luciferase reporter assay was used to determine the transcription activity of MTP. ChIP-PCR was performed to confirm the specific FoxO1 binding region of MTP promoter. Results Palmitate treatment induced MIN6 cells apoptosis. Compared with control, the mRNA level of MTP was significantly elevated in palmitate treated MIN6 cells and primary islets isolated from 4 , 8-week db/db mice. Luciferase reporter assay showed that the transcription of MTP was positively regulated by FoxO1. ChIP-PCR confirmed the binding of FoxO1 to the specific region of MTP promoter. Conclusion MTP is positively regulated by transcriptional factor FoxO1 in palmitate-induced pancreatic β cell apoptosis.关键词
胰岛β细胞/脂肪酸/FoxO1/转录调控/微粒体甘油三脂转移蛋白Key words
pancreatic β cell/ FoxO1/ transcription regulation/ MTP分类
生物科学引用本文复制引用
宣慧,林海燕,张洁心,朱云霞,韩晓..脂肪酸诱导胰岛β细胞凋亡过程中FoxO1对MTP的转录调控研究[J].医学分子生物学杂志,2011,8(4):289-293,5.基金项目
国家重点基础研究发展规划项目(973计划)(No.20-11CB504003),国家自然科学基金(No.30900542),江苏省"333高层次人才培养工程"基金(2010年) （973计划）
