渔业科学进展2011，Vol.32Issue(3)：26-32,7.
10种软骨鱼线粒体基因组特征分析
Analysis of the characteristics of ten cartilaginous fishes mitochondrial genomes
摘要
Abstract
The basic characteristics of chondrichthyes mitochondrial genomes were fully revealed by comprehensive analysis of mitochondrial genomes in ten cartilaginous fishes.Mitochondrial genomes of cartilaginous fishes contain 37 standard metazoan genes and their gene order is identical.The Ka/Ks ratio of 13 mitochondrial protein-coding genes in the Carcharhiniformes and Rajiformes classes is much lower than 1 (0.019 1～0.156 4), indicating a strong purifying selection (negative selection).The phylogenetic trees based on mitochondrial genomes showed that the class Chondrichthyes is divided into two clades: the subclass Holocephali and the subclass Elasmobranchii.Inside the subclass Elasmobranchii, the order Rajiformes and the order Myliobatiformes were clustered as a clade, and the other five orders (Carcharhiniformes, Lamniformes, Orectolobiformes, Heterodontiformes, and Squaliformes) were clustered as another clade.The phylogenetic relationship is (((Carcharhiniformes + Lamniformes) + Orectolobiformes) + Heterodontiformes) +Squaliformes.The genetic variation analysis of main genes (13 protein coding genes and 2 ribosomal RNA genes) among cartilaginous fish species showed that nad5 and nad4 genes are ideal molecular markers and can be used as supplementary molecular markers to coxl gene,which can be used to analyze the genetic diversity among different groups and provide more basis for rational use of its biological resources.关键词
软骨鱼纲/线粒体基因组/系统发育/差异位点/分子标记Key words
Chondrichthyes/ Mitochondrial genome/ Phylogeny/ Genetic different loci/Molecular marker分类
生物科学引用本文复制引用
申欣,田美,孟学平,程汉良..10种软骨鱼线粒体基因组特征分析[J].渔业科学进展,2011,32(3):26-32,7.基金项目
国家自然科学基金(40906067)、江苏省"青蓝工程"人才基金(苏教师[2010]27号)、江苏省自然科学基金(BK2007066)、江苏省海洋生物技术重点建设实验室研究基金(2009HS13)和淮海工学院自然科学基金(Z2009048)共同资助 （40906067）
