渔业科学进展2011，Vol.32Issue(3)：111-115,5.
PCR结合变性高效液相色谱快速检测发酵乳酸杆菌
Rapid detection of Lactobacillus fermentum by PCR-DHPLC
摘要
Abstract
Rapid detection of Lactobacillus fermenturn was established by using polymerase chain reaction (PCR) and denaturing high-performance liquid chromatography (DHPLC).The primers were designed and the PCR system was optimized with the elongation factor (EFTu) of L. fermentum as the target gene. Twenty-six strains including L. gasseri were tested with specific detection. The sensitivity of various diluted standard strains were determined. The results indicated that the PCR-DHPLC method was specific and sensitive with a detection limit of 100 CFU/ml.关键词
发酵乳酸杆菌/PCR/变性高效液相色谱/检测Key words
Lactobacillus fermenturn/ PCR/ DHPLC/ Detection分类
化学化工引用本文复制引用
杨大伟,周裔彬,刘云国,雷质文,陈俊芳,王建广,李正义..PCR结合变性高效液相色谱快速检测发酵乳酸杆菌[J].渔业科学进展,2011,32(3):111-115,5.基金项目
国家质检总局课题(2008IK140 （）
2009IK170)资助 （）
