中国病理生理杂志2011，Vol.27Issue(8)：1597-1602,6.DOI:10.3969/j.issn.1000-4718.2011.08.026
胆红素和内毒素联合作用对NRK52E细胞缝隙连接功能的影响
Function of gap junction in NRK52E cells exposed to bilirubin combined with lipopolysaccharide
田野 1庞红宇 2孙国亮 2李晓芸 2位静 2黑子清2
作者信息
- 1. 中山市人民医院麻醉科,广东,中山,528403
- 2. 中山大学附属第三医院麻醉科,广东,广州,510630
- 折叠
摘要
Abstract
AIM: To observe the effect of bilirubin ( BR ) combined with lipopolysaccharide ( LPS ) on the growth and gap junction of NRK52E cells.METHODS: NRK52E cells were cultured and treated with different concentrations of bilirubin combined with lipopolysaccharide.The growth of NRK52E cells was measured by MTT method.The function of gap junction in NRK52E cells was determined by the method of fluoroimmunoassay.RESULTS: BR promoted the growth of NRK52E cells in a dose - dependent manner from 17.1 μmol/L to 513 μmol/L but inhibited it at more than 513 μmol/L.LPS inhibited the growth of NRK52E cells in a dose - dependent manner from 10-1 000 μg/L.Under the condition of BR combined with LPS at 100 μg/L, BR at 513 μmol/L increased the growth of NRK52E cells while BR at 684 μmol/L decreased the growth of the cells ( P ＜0.05 ).Combined with LPS at 100 μg/L, BR at 513 μmol/L increased the permeability of the gap junction ( P ＜ 0.05 ), while BR at 684 μmol/L decreased the permeability of the gap junction.CONCLUSION: BR at a low concentration ( 513 μmol/L ) decreases, and BR at a high concentration ( 684 μmol/L ) increases the toxicity of LPS on NRK52E cells by affecting the function of gap junctions in the cells.关键词
胆红素/脂多糖类/NRK52E细胞/缝隙接合部Key words
Bilirubin/ Lipopolysaccharides/ NRK52E cells/ Gap junctions分类
医药卫生引用本文复制引用
田野,庞红宇,孙国亮,李晓芸,位静,黑子清..胆红素和内毒素联合作用对NRK52E细胞缝隙连接功能的影响[J].中国病理生理杂志,2011,27(8):1597-1602,6.
