中国病理生理杂志2011，Vol.27Issue(8)：1485-1489,5.DOI:10.3969/j.issn.1000-4718.2011.08.006
血管紧张素Ⅱ和血管紧张素-(1-7)对大鼠血管平滑肌细胞肾素(原)受体表达的影响
Effects of angiotensinⅡ and angiotensin-(1-7) on expression of(pro) renin receptor in rat vascular smooth muscle cells
摘要
Abstract
AIM: To observe the effects of angiotensin Ⅱ ( Ang Ⅱ ) and angiotensin - ( 1 - 7 ) [Ang - ( 1 -7 )]on the expression of ( pro )renin receptor [( P )RR]in rat vascular smooth muscle cells.METHODS: The cultured VMSCs were randomly divided into control group, Ang Ⅱ group, Ang - ( 1 - 7 ) group, Ang Ⅱ + losartan ( an AT1 receptor antagonist) group, Ang Ⅱ + PD123319( an AT2 receptor antagonist ) group and CGP42112A ( an AT2 receptor agonist ) group.The expression of ( P )RR at protein and mRNA levels was detected by Western blotting and real - time PCR, respectively.RESULTS: Compared with control group, Ang Ⅱ distinctly increased and Ang - ( 1 - 7 ) decreased the expression of ( P )RR mRNA and protein in VMSCs in a dose - dependent manner ( P ＜ 0.01 ).Compared with Ang Ⅱ group, losartan did not inhibit the expression of ( P ) RR mRNA and protein in VMSCs induced by Ang Ⅱ ( P ＞ 0.05 ), but PD123319 did ( P ＜ 0.01 ).CGP42112A also induced the expression of ( P )RR protein and mRNA in VMSCs ( P ＜ 0.01 ).CONCLUSION: Ang I induces the expression of ( P )RR in VMSCs by AT2.However, Ang - ( 1 - 7 ) inhibits the expression of ( P )RR in VMSCs.关键词
肾素(原)受体/血管紧张素-(1-7)/血管紧张素Ⅱ/血管平滑肌细胞Key words
( Pro )rorenin receptor/ Angiotensin - ( 1 - 7 )/ Angiotensin Ⅱ / Vascular smooth muscle cells分类
医药卫生引用本文复制引用
张娜娜,李茂莲,边云飞,高奋,杨志明,杨慧宇,肖传实..血管紧张素Ⅱ和血管紧张素-(1-7)对大鼠血管平滑肌细胞肾素(原)受体表达的影响[J].中国病理生理杂志,2011,27(8):1485-1489,5.基金项目
山西省回国留学人员科研资助项目(No.2011-110) （No.2011-110）
