中国组织工程研究与临床康复2011，Vol.15Issue(27)：4988-4992,5.DOI:10.3969/j.issn.1673-8225.2011.27.011
猪脂肪干细胞与脱细胞真皮基质的生物相容性
Biocompatibility of procine adipose derived stem cells and acellular dermal matrix
摘要
Abstract
BACKGROUND: Synthetic osteogenic growth peptide can stimulate proliferation and osteoblast differentiation of bonemesenchymal stem cells (BMSCs) in vitro, and which can enhance bone density in vivo, but the mechanism of the effect ofosteogenic growth peptide (OGP) on BMSCs is not clear yet.OBJECTIVE: To investigate the effect of OGP on gene expression changes in BMSCs using mouse whole-genome microarrayand to explore the gene and signal transduction pathway influenced by OGP.METHODS: Mouse whole-genome Oligo chip was obtained from Shanghai Bo Hao Biotechnology Co., Ltd., gene expressionchanges in BMSCs of OPG-/- in the OGP group (treated with OGP) and control group were screened. Real-time quantitative reverse transcriptase-polymerase chain reaction (RT -PCR) was used to analyze and verify gene expression changes related to proliferation and differentiation, and which combined with cluster analysis and pathway analysis to explore the mechanism of OPG.RESULTS AND CONCLUSION: Chip results showed that 346 genes expression down -regulated by OPG, 121 genes expression up-regulated. PCR validation results were consistent with the chip. Six pathways proteins were identified by BioCarta pathway analysis and 12 pathways were identified by KEGG pathway analysis. Multiple pathways were related to the mechanism of the effect of OGP on gene expression changes in BMSCs of OPG-/- mice, among which the MAPK signal transduction pathway may play an important role in their prolifera tion.关键词
脱细胞真皮基质/脂肪源性干细胞/酶消化法/培养/生物相容性/猪/组织工程分类
医药卫生引用本文复制引用
赵宝成,王振军,韩加刚,顾蓓,徐慧民,喻学桥..猪脂肪干细胞与脱细胞真皮基质的生物相容性[J].中国组织工程研究与临床康复,2011,15(27):4988-4992,5.基金项目
北京市卫生系统高层次卫生技术人才(2009-1-03) （2009-1-03）
国家级新世纪百千万人才工程(08-009). （08-009）
