中国组织工程研究与临床康复2011，Vol.15Issue(27)：5058-5062,5.DOI:10.3969/j.issn.1673-8225.2011.27.026
基质细胞衍生因子1趋化神经干细胞迁移的体外效应
In vitro effect of stromal cell derived factor -1 on the migration of neural stem cells
摘要
Abstract
BACKGROUND: Neural stem cell migration plays important roles in the development and repair of the central nervous system.Although recent studies have shown that the chemokines mediate neural stem cell migration, but the mechanism is unclear.OBJECTIVE: To explore the effects of stromal cell-derived factor-1 (SDF-1) on migration of fetal rat hippocampus neural stem cells in vitro.METHODS: The fetal rat hippocampal neural stem cells were isolated, cultured and identified in serum-free medium. The expression of CXCR4 in neural stem cells was detected using immunofluorescence and RT-PCR. The under-agarose cell migration assay was used to observe the effects of SDF-1(50-500 ng/ml) on neural stem cell migration, and blocking CXCR4, the receptor of SDF-1, to identify the specificity of migration.RESULTS AND CONCLUSION: Immunofluorescence results showed that neural stem cells were CXCR4 positive, and RT-PCR findings showed 643 bp specific band in agarose gel electrophoresis. The Under-Agarose cell migration assay showed that: SDF-1 (50-500 ng/ml) could accelerate the migration of neural stem cells, the migration increased with the concentration, and 500 μg/L was the best. By adding anti -CXCR4 polyclonal antibodies, the migration of neural stem cells compared with SDF-1 was significantly reduced, no significant difference with the control group (P ＞ 0.05), pointing out anti -CXCR4 polyclonal antibodies can block the effect of the migration.关键词
基质细胞衍生因子1/神经干细胞/趋化因子/细胞迁移/CXCR4分类
基础医学引用本文复制引用
牟临杰,丁鹏,王崇谦,王伟民,李宣鹏,王进昆..基质细胞衍生因子1趋化神经干细胞迁移的体外效应[J].中国组织工程研究与临床康复,2011,15(27):5058-5062,5.基金项目
2008 年云南应用基础研究面上项目(2008ZC132M). （2008ZC132M）
