中国临床保健杂志2011，Vol.14Issue(4)：395-397,3.DOI:10.3969/J.issn.1672-6790.2011.04.022
血必净对脂多糖诱导的急性肺损伤大鼠肺组织高迁移率族蛋白1表达的影响
The effect of Xuebijing on expression of HMGB1 in lipopolysaccharide-induced acute lung injury in rats
张之龄 1尹小燕 1童小文 1朱健1
作者信息
- 1. 上海交通大学医学院附属第九人民医院急诊科,上海,200011
- 折叠
摘要
Abstract
Objective To study high mobility group box 1 (HMGB1)expression in lipopolysaccharide-induced acute lung injury in rats and investigate the effect of Xuebijing and its possible mechanism. Methods 45 Wistar rats were randomly divided into 3 groups as following: ( 1 ) control group ( n = 15 ), ( 2 ) LPS group ( n = 15 ), ( 3 )Xuebijing treatment( XBJ )group( n = 15 ). LPS(5mg/kg)was infused by femoral vein to reproduce ALl model in LPS group and XBJ group. Male Wistar rats were given either saline in LPS group or XBJ in XBJ group 4ml/kg 30 min before the intravenous injection of a bolus of LPS, whereas control group were given equivalent volume normal saline.Five rats in each groupwere killed at 6h,12 h and 24 h,which were divided into subgroup Ⅰ, Ⅱ, Ⅲ ,and lung tissue was collected. The pathomorphism ohanges of lung tissue were observed using light microscope. and the expression of HMGB1 protein in pulmonary was examined by immunohistochemistry in all the rats. Results The expression of HMGB1 protein in pulmonary showed a gradual increase and reached the peak at 24h in LPS group. Lower level of HMGB1 protein was found in XBJ group than that in LPS group at all time points( P ＜0.05 ). The pathological change was more obvious in LPS group than that in XBJ group. Conclusion HMGB1 exipression is increased in acute lung injury in the rats induced by LPS. Xuebijing could attenuate the degree of acute lung injury in rats induced by LPS,which may be related to its inhibition of the HMGB1 protein expression in lung tissue.关键词
呼吸窘迫综合征,成人/脂多糖类/高纤移率族蛋白质类/中药疗法Key words
Respiratory distress syndrome/ adult/ Lipopolysaccharides/ High mobility group proteins/ Drug therapy (TCD)分类
医药卫生引用本文复制引用
张之龄,尹小燕,童小文,朱健..血必净对脂多糖诱导的急性肺损伤大鼠肺组织高迁移率族蛋白1表达的影响[J].中国临床保健杂志,2011,14(4):395-397,3.
