中国组织工程研究与临床康复2011，Vol.15Issue(28)：5157-5160,4.DOI:10.3969/j.issn.1673-8225.2011.28.007
雌激素受体基因ERβ与小鼠成骨细胞的增殖和分化
Estrogen receptor beta inhibits the proliferation and differentiation of osteoblasts in mice
摘要
Abstract
BACKGROUND: Estrogen receptors (ERs) are expressed in cell components related to bone formation and resorption.OBJECTIVE: To investigate the regulatory effects of Er? on osteoblast proliferation and differentiation.METHODS: The mouse osteoblastic cell lines MC3T3-E1 were divided into 3 groups: experimental group (transfected ER?RNAi vector), negative control (transfected ERL RNAi vector), and blank control (no transfection). Three groups of cells were cultured under the same conditions. In each group, cell growth curve was drawn using MTT; and the cell cycle was analyzed by flow cytometry.RESULTS AND CONCLUSION: The cell proliferation in the experimental group was more significantly enhanced than in the negative control group or blank control group. The percentage of cells at G1-phase in the experimental group was significantly less than the other groups, while the percentage of cells at S-phase and G2-phase was significantly higher than the other groups (P ＜ 0.05). According to test results of the ER? silence, we can infer that the ER? may inhibit the proliferation and differentiation of osteoblasts.关键词
成骨细胞/雌激素受体/RNAi/增殖/分化分类
基础医学引用本文复制引用
鲁世金,张宏其,高其乐,郭超峰,唐明星..雌激素受体基因ERβ与小鼠成骨细胞的增殖和分化[J].中国组织工程研究与临床康复,2011,15(28):5157-5160,4.基金项目
湖南省研究生科研创新项目资助(CX2010B101). （CX2010B101）
