中国组织工程研究与临床康复2011，Vol.15Issue(28)：5217-5220,4.DOI:10.3969/j.issn.1673-8225.2011.28.022
复制缺陷型腺病毒载体介导人血管内皮细胞生长因子cDNA促进静脉分叶皮瓣的成活
Replication-defective adenovirus vector-mediated human vascular endothelial cell growth factor cDNA promotes survival of lobulated venous flap
摘要
Abstract
BACKGROUND: Lobulated venous flap has some unique advantages to repair the soft tissue defects in many fingers. However,the poor viability of venous flaps limits their clinical applications.OBJECTIVE: To explore the effects of replication-defective adenovirus vector-mediated human vascular endothelial cell growth factor (VEGF) gene on survival of lobulated venous flap.METHODS: 24 rabbits were randomly divided into three groups. Lateral abdominal wall skin flap was created in rabbits. At 5 days before operation, Ad-VEGF165 (Ad-VEGF165 group), Ad-Gal (Ad-Gal group) and normal saline (NS group) were subcutaneously injected in the skin flap. The survival rate of the flap and the number of the new blood vessels were determined on the 7th day after operation.RESULTS AND CONCLUSION: The flap survival rate was (76.281±2.298)％, (50.408±1.577)％ and (48.748±2.130)％ in the Ad-VEGF165, Ad-Gal and NS groups, respectively. The number of the new blood vessels was (37.76±2.21) cm2, (22.68±2.15) cm2,and (23.31±3.38)/cm2 in the above-mentioned three groups, respectively. Immunohistochemical staining showed that lots of VEGF was expressed in the Ad-VEGF165 group. Blood vessel hyperplasia was obvious in the Ad-VEGF165 group. The replication-defective adenovirus vector-mediated VEGF gene can increase the neovascularization in lobulated venous flap and augments their survival rate.关键词
静脉皮瓣/血管内皮细胞生长因子/基因治疗/腺病毒/分叶皮瓣/组织构建分类
医药卫生引用本文复制引用
谢红炬,邓颖,李明,林彪斌,陈碾..复制缺陷型腺病毒载体介导人血管内皮细胞生长因子cDNA促进静脉分叶皮瓣的成活[J].中国组织工程研究与临床康复,2011,15(28):5217-5220,4.基金项目
课题受湖南省卫生厅资助课题(B2006-117)资助. （B2006-117）
