中国组织工程研究与临床康复2011，Vol.15Issue(29)：5339-5342,4.DOI:10.3969/j.issn.1673-8225.2011.29.005
丝素蛋白/羟基磷灰石材料复合骨髓间充质干细胞构建组织工程化软骨
Silk fibroin/hydroxyapatite combined with bone marrow mesenchymal stem cells for construction of tissue engineered cartilage
杨耀 1徐卫袁 2张亚 3张兴祥 2刘宗宝 2钱辉 2黄建平 2崔志浩2
作者信息
- 1. 江苏大学临床医学院,江苏省镇江市,212013
- 2. 张家港市第一人民医院骨科,江苏省张家港市,215600
- 3. 苏州大学附属儿童医院,江苏省苏州市,215003
- 折叠
摘要
Abstract
BACKGROUND: With the emergence of tissue engineering, the possibility of repairing the cartilage injury is greatly increased,but single scaffold materials fail to meet ideal scaffold, and have some limitations.OBJECTIVE: To explore the feasibility of silk fibroin/hydroxyapatite combined with bone marrow mesenchymal stem cells (BMSCs) for construction of tissue engineered cartilage METHODS: BMSCs were isolated, cultured and induced into chondrocytes, which were cocultured with silk fibrion/hydroxyapatite to construct full-thickness articular cartilage defects in the platform of knee joints tibial plateau. A total of 45 rabbits with unilateral knee full thickness cartilage defects model were randomly divided into 3 groups. The compound group was implanted with cell-silk fibrion/hydroxyapatite compound; material group was only implanted with silk fibrion/hydroxyapatite;implantation was not given in the control group. The reparation conditi on of cartilage defects was observed by CT examination and histological examination at 8, 12 weeks after implantation.RESULTS AND CONCLUSION: In the compound group, the articular surface was not flat, the joint space was increased, formed new chondrocyte-like cells and extracellular matrix was extremely rich after 8 weeks. In the material group, articular surface was collapsed and a small amount of chondrocytes were proliferated. After 12 weeks, in the compound group, the articular surface was flat, the joint space was normal; a large number of chondrocytes were emerged, and the color of repaired tissues was closed to surrounding cartilage and scaffolds were completely degraded. In the material group, the articular surface was not flat and cartilage cells did not completely filled, some scaffolds were degraded. The defects were not repaired in the control group. It is indicated that the method of repairing the full-thickness hyaline cartilage defects using BMSCs combined with silk fibroin/hydroxyapatite is feasible, and the silk fibroin/hydroxyapatite can be used as scaffold materials in articular cartilage tissue engineering due to a good biocompatibility.关键词
丝素蛋白/羟基磷灰石材料/骨髓间充质干细胞/细胞诱导/软骨缺损/软骨组织工程分类
基础医学引用本文复制引用
杨耀,徐卫袁,张亚,张兴祥,刘宗宝,钱辉,黄建平,崔志浩..丝素蛋白/羟基磷灰石材料复合骨髓间充质干细胞构建组织工程化软骨[J].中国组织工程研究与临床康复,2011,15(29):5339-5342,4.
