基础医学与临床2011，Vol.31Issue(9)：970-975,6.
蛇血清分泌型磷脂酶A2抑制剂防治人炎症的生物信息学分析
Bioinformatics analysis of possible inhibition of human inflammation by secretory phospholipase A2 inhibitor from snake serum
摘要
Abstract
Objective To compare the structural similarities of human secretory phospholipase A2 (sPLA2) with snake venom sPLA2, and to analyze the neutralization effect of sPLA2 inhibitors (PLIs) from snake serum against sPLA2 by exploring their relationship between structure and function, which was meaningful for assessing PLIs' clinical application in inflammation protection. Methods Multiple sequencial alignment of 7 human sPLA2 and 3 fivepace snake venom sPLA2 was made by Clustal W2. Tertiary structures of the 10 sPLA2 were predicted by SwissModelling. Spatial structure similarity between snake venom and human sPLA2 was assessed by UCSF Chimera. Results Human and snake venom sPLA2 shared 40％ sequencial homologous and approximately similar spatial structures. All the PLIs (Υtype) have similar tertiary charectristics and conserved binding region to sPLA2. Conclusion Based on the bioinformatical analysis, snake serum PLls were found to be an effective inhibitor to snake venom toxicity and human sPLA2 activity. Thus, the PLIs were potential blockers to human sPLA2 inflammation pathway and may be used for clinical disease.关键词
磷脂酶A2/磷脂酶A2抑制剂/生物信息学Key words
phospholipase A2/ phospholipase A2 inhibitor/ bioinformatics分类
医药卫生引用本文复制引用
陈柯,邓欣如,徐曦,李晓,钟立鹏,黄春洪..蛇血清分泌型磷脂酶A2抑制剂防治人炎症的生物信息学分析[J].基础医学与临床,2011,31(9):970-975,6.基金项目
国家大学生创新实验计划(091040303) （091040303）
国家自然科学基金(30860347) （30860347）
