基础医学与临床2011，Vol.31Issue(9)：976-979,4.
重组腺病毒Ad-ING4和Ad-PTEN抑制肝癌细胞增殖
Investigation of inhibition of adenovims mediated gene ING4 and PTEN to hepatocarcinoma cells
摘要
Abstract
Objective We use recombinant Ad-ING4 and Ad-PTEN to infect human hepatocarcinoma cell line SMMC-7721 in vitro to test and compare the effects of gene expression of the cells. Methods We use recombinant Ad-ING4 and Ad-PTEN and control virus to infect QBI-293A cells for viral propagation and viral titration. The infective effects of Ad-ING4, Ad-PTEN and control virus were confirmed by fluorescence microscopy. Use Ad-ING4 and Ad-PTEN to infect SMMC-7721 cells and confirm their effects by their apoptotic rate and the results of MTT.Results The apoptotic rate of control virus group is 3.4％ ± 1.3％, the Ad-ING4 group is 49. 7％ ±4. 8％ (P ＜ 0. 01 )and the Ad-PTEN group is 11.4％ ± 3.2％ (P ＜ 0. 01 ). The cell proliferation of group Ad-ING4 and AdPTEN obviously decreased from 48h to 96h( P ＜0.05 ). Conclusion The data analysis showed Ad-ING4 and AdPTEN had an inhibitory effect to SMMC-7721 cells especially that of Ad-ING4.关键词
肝癌/Ad-ING4/Ad-PTEN/基因Key words
hepatocarcinoma/ Ad-ING4/ Ad-PTEN/ gene分类
医药卫生引用本文复制引用
姚雷,曹婧媛,吴鹏飞,黄玉洪,王金志,杨吉成,缪竞诚..重组腺病毒Ad-ING4和Ad-PTEN抑制肝癌细胞增殖[J].基础医学与临床,2011,31(9):976-979,4.基金项目
国家级大学生创新性实验计划项目(SG315859) （SG315859）
