基础医学与临床2011，Vol.31Issue(9)：1015-1020,6.
乌司他丁对急性肺损伤大鼠TNF-α、IL-10mRNA及P38MAPK表达的影响
Effect of ulinastatin on mRNA of cytokine and P38 MAPK expression of rats with acute lung injury
张新颖 1刘奉琴 1刘海燕 1刘玲 2程红霞 3王伟 1王玉林1
作者信息
- 1. 山东大学,附属省立医院,儿科,山东,济南,250021
- 2. 山东大学,第二医院,临床分子生物学实验室,山东,济南,250033
- 3. 山东大学,附属省立医院,病理科,山东,济南,250021
- 折叠
摘要
Abstract
Objective To explore the protective effect of ulinastatin (UTI) in a rat model of lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the underlying molecular mechanism.Methods Wistar rats were randomly assigned into: control group, model group (LPS 5 mg/kg, iv ), and intervention group (UTI 50 000 U/kg, iv).Expression of TNF-alpha and IL-10 mRNA in lung tissue were measured by real time RT-PCR.Expression of phosphorylated P38 MAPK in lung tissue were detected by immunohistochemical staining and Western blot methods.Results Expression of TNF-alpha mRNA at 0.5,1 and 3 h in rats of model group is 78.55 ± 18.99,128.74 ± 34.79 and 12.29 ± 1.32 folds against control group, which decreased to 20.95 ± 1.45 ( P ＜ 0.01 ),58.15 ± 11.01 (P ＜0.0l ) and 2.85 ±0.57(P ＜0.05 ) folds in intervention group.Expression of IL-10 mRNA at 0.5,1 and 3 h in rats of model group is 20.89 ±4.60,38.20 ±8.26 and 53.26 ±8.01 folds against control group,which increased to 66.77 ± 11.18 ( P ＜ 0.05 ) ,97.69 ± 27.00 ( P ＜ 0.01 ) and 128.62 ± 42.30 ( P ＜ 0.01 ) folds against invention group.Administration of LPS elevated the expression of P38 MAPK, which were significantly attenuated by UTI at each time point( ( P ＜ 0.05 ).Conclusion UTI may attenuate ALI by regulating the gene expression of eytokines.P38 MAPK played role in the decreasing of TNF-alpha mRNA by UTI.关键词
乌司他丁/急性肺损伤/肿瘤坏死因子/白细胞介素/P38 MAPKKey words
ulinastatin/ acute lung injury/ tumor necrosis factor/ interleukin/ P38 mitogen-activated protein kinase分类
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张新颖,刘奉琴,刘海燕,刘玲,程红霞,王伟,王玉林..乌司他丁对急性肺损伤大鼠TNF-α、IL-10mRNA及P38MAPK表达的影响[J].基础医学与临床,2011,31(9):1015-1020,6.
