中国组织工程研究与临床康复2011，Vol.15Issue(27)：4975-4978,4.DOI:10.3969/j.issn.1673-8225.2011.27.008
基因芯片分析成骨生长肽对OPG-/-小鼠骨髓间充质干细胞的作用
Effect of osteogenic growth peptide on gene expression changes in bone mesenchymal stem cells of OPG- /- mice: A Microarray analysis
米尔萨力江·亚森 1郭常军 1费琴明 1陈统一 1崔大敷2
作者信息
- 1. 复旦大学附属中山医院骨科,上海市,200032
- 2. 中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海市,200031
- 折叠
摘要
Abstract
BACKGROUND: Synthetic osteogenic growth peptide can stimulate proliferation and osteoblast differentiation of bone mesenchymal stem cells (BMSCs) in vitro, and which can enhance bone density in vivo, but the mechanism of the effect of osteogenic growth peptide (OGP) on BMSCs is not clear yet. OBJECTIVE: To investigate the effect of OGP on gene expression changes in BMSCs using mouse whole-genome microarray and to explore the gene and signal transduction pathway influenced by OGP.METHODS: Mouse whole-genome Oligo chip was obtained from Shanghai Bo Hao Biotechnology Co., Ltd., gene expression changes in BMSCs of OPG-/- in the OGP group (treated with OGP) and control group were screened. Real-time quantitative reverse transcriptase-polymerase chain reaction (RT -PCR) was used to analyze and verify gene expression changes related to proliferation and differentiation, and which combined with cluster analysis and pathway analysis to explore the mechanism of OPG.RESULTS AND CONCLUSION: Chip results showed that 346 genes expression down -regulated by OPG, 121 genes expressionup-regulated. PCR validation results were consistent with the chip. Six pathways proteins were identified by BioCarta pathway analysis and 12 pathways were identified by KEGG pathway analysis. Multiple pathways were related to the mechanism of the effect of OGP on gene expression changes in BMSCs of OPG-/- mice, among which the MAPK signal transduction pathway may play an important role in their proliferation.关键词
基因芯片/骨髓间充质干细胞/成骨生长肽/信号传导/细胞增殖分类
基础医学引用本文复制引用
米尔萨力江·亚森,郭常军,费琴明,陈统一,崔大敷..基因芯片分析成骨生长肽对OPG-/-小鼠骨髓间充质干细胞的作用[J].中国组织工程研究与临床康复,2011,15(27):4975-4978,4.
