中国组织工程研究与临床康复2011，Vol.15Issue(27)：4979-4983,5.DOI:10.3969/j.issn.1673-8225.2011.27.009
多因子联合诱导脂肪组织源性干细胞分化为神经元样细胞
Multiple factors-induced differentiation of rat adipose tissue-derived stem cells into neuron-like cells
摘要
Abstract
BACKGROUND: Differentiation of adipose tissue-derived stem cells (ADSCs) into neurons has been reported. However, little data are available regarding differential adhesion-purified ADSCs differentiation into neuron-like cells induced by multiple factors. OBJECTIVE: To explore the feasibility of inducing rat ADSCs into neuron-like cells with a new scheme and medium containing multiple factors.METHODS: ADSCs were isolated from the fat pads in groins and epididymis of male Sprague Dawley rats by enzyme digestion,and purified by differential attachment method. Fluorescent immunocytochemistry was used to identify the phenotype of the cells (CD44, CD49d and CD106 expressions). Differentiation of ADSCs into neuron-like cells was induced in cocktail-like neuronal differential medium containing insulin, hydrocortisone, valproic acid and forskolin. Also, fluorescent immunocytochemistry was used to identify the markers of the differentiated cells (Nestin and NeuN).RESULTS AND CONCLUSION: After sequentially isolated, purified and cultured, the obtained cells presented with long-fusiform shape, with clear nucleoli and light stained cytoplasm. They were positive for CD44 and CD49d, but negative for CD106. The application of NIM resulted in morphological changes of ADSCs during the first 6 hours, shown by pyramidal, irregular or triangular cell body with short, thin finger-like cell processes. After 24 hours of induction, most of the cells developed multiple branched processes resembling those of neurons and positive for Nestin and NeuN. Results showed that ADSCs could be induced to differentiate into neuron-like cells in cocktail-like neuronal differential medium.关键词
脂肪组织源性干细胞/分化/神经元/免疫细胞化学/大鼠分类
医药卫生引用本文复制引用
张丽华,张易,汤银娟,董为人,郭家松,陈小艳,王海红,陈盼盼,叶泉英,冯淑兰..多因子联合诱导脂肪组织源性干细胞分化为神经元样细胞[J].中国组织工程研究与临床康复,2011,15(27):4979-4983,5.基金项目
佛山市卫生局医学科研立项课题(2010177) （2010177）
佛山市科技局医学类科技攻关项目(201008063) （201008063）
广东省高校优秀中青年人才基金资助(C1010086) （C1010086）
广东省自然科学基金资助 （）
