姜黄素对小鼠心脏发育相关基因表达的影响及其表观遗传调控机制OA北大核心CSCDCSTPCD
Effects of curcumin on heart development-related genes expression and the associated epigenetic mechanism
目的 阐明姜黄素对心脏发育相关基因GATA4,MEF2C和Nkx2.5表达的影响,探讨其表观调控机制.方法 姜黄素处理新生鼠心肌细胞后,分别用ELISA,Western blot,real time RT-PCR和染色质免疫沉淀(ChIP)检测组蛋白乙酰化酶活性,组蛋白H3乙酰化修饰状态,GATA4,MEF2C和Nkx2.5的表达和启动子区域组蛋白乙酰化水平.结果 30μmol/L姜黄素作用24 h能有效降低心肌细胞中组蛋白乙酰化酶活性和组蛋白H…查看全部>>
Objective To investigate the effects of curcumin on heart development-related genes GATA4, MEF2C and Nkx2.5, and to explore the epigenetic regulation mechanism.Methods Cardiocytes from neonatal mouse were treated with curcumin with different concentrations and different time.ELISA was used to detect the histone acetylase(HATs) activity of cardiocytes, Western blot was used to detect the acetylation of histone H3 and real time RT-PCT to measure the expression…查看全部>>
孙慧超;朱静;吕铁伟;吴晓云;黄国英;田杰
重庆医科大学附属儿童医院,心脏内科儿童发育疾病研究省部共建教育部重点研究室,重庆,400014儿童发育疾病研究省部共建教育部重点研究室,重庆,400014重庆医科大学附属儿童医院,心脏内科重庆医科大学附属儿童医院,心脏内科复旦大学附属儿童医院,心脏中心,上海,201102
医药卫生
组蛋白乙酰化姜黄素P300表观遗传学心肌细胞
histone acetylation curcumin P300 epigenetics cardiocyte
《基础医学与临床》 2011 (9)
HATs亚型对心脏发育相关基因表达的时序调控
959-964,6
973基金资助(2010CB529505)国家自然科学基金资助(30770889)
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