中国组织工程研究与临床康复2011，Vol.15Issue(28)：5221-5224,4.DOI:10.3969/j.issn.1673-8225.2011.28.023
血管内皮生长因子及其受体在大鼠附睾内精子上的表达
Expression of vascular endothelial growth factor and its receptor in the epididymal sperm of adolescent male rats
摘要
Abstract
BACKGROUND: Vascular endothelial growth factor (VEGF) and its receptor fms-like tyrosine kinase-1 (Flt-1) have a very important position in the field of male reproduction. However, it is still unclear about their expression meaning and regulatory mechanism in the reproductive system. OBJECTIVE: To study the expression and location of VEGF and Flt-1 in the epididymal sperm of adolescent male rats. METHODS: The expression of VEGF and Flt-1 was detected in 10 adolescent SD rats. The concentration of the sperm was (30-40)×109/L. Immunofluorescent staining was used for VEGF and Flt-1 expression and location in the sperm. RESULTS AND CONCLUSION: Immunofluorescent staining showed that VEGF and Flt-1 were both localized in the acrosome of sperm head, as well as in the neck, middle and principal segment of sperm tail. Specific expression patterns of VEGF and Flt-1 in the sperm of rats display that they may participate in spermiotelcosis, relevant to movement, capacitation and acrosome reaction of the sperm.关键词
血管内皮生长因子/受体/类fms酪氨酸激酶/精子/免疫荧光分类
基础医学引用本文复制引用
艾庆燕,赵豫凤,王艳梅,苗乃周,马莉,杨加周..血管内皮生长因子及其受体在大鼠附睾内精子上的表达[J].中国组织工程研究与临床康复,2011,15(28):5221-5224,4.基金项目
陕西省科技厅自然科学基础项目(2010JM4024) （2010JM4024）
陕西省教育厅专项科研计划项目(2010JK906,2010JK907) （2010JK906,2010JK907）
延安市科学技术研究发展计划项目(2009KS-12) （2009KS-12）
延安大学科研计划项目(YD2008-04).the Natural Science Foundation of Shaanxi Science and Technology Bureau,No.2010JM4024 （YD2008-04）
Specific Science and Technology Research Plan of Shaanxi Education Bureau,No.2010JK906,2010JK907 （）
Science and Technology Research Development Plan of Yan'an City,No.2009KS-12 （）
Science and Technology Research Plan of Yan'an University,No.YD2008-04 （）
