基础医学与临床2011，Vol.31Issue(9)：1006-1010,5.
E-钙黏蛋白siRNA促进胃癌细胞SGC7901耐药与增殖
E-cadherin siRNA promotes development of drug resistance and proliferation in gastric cancer cell line SGC7901
陈勇 1刘颖 1高鸿 1刘巍1
作者信息
- 1. 河北医科大学,第四医院,肿瘤内科,河北,石家庄050011
- 折叠
摘要
Abstract
Objective To explore the effect of E-cadherin siRNA on drug sensitivity and cell proliferation and their related mechanisms in SGC7901 cell line. Methods SGC7901 cell line was transfected with E-cadherin siRNA by means of Lipofectamine 2000. Drug sensitivity of transfected cells to CDDP,5-FU, paclitaxel and ADR was tested using MTT assay. And proliferation changes of cells were detected after transfection. Protein changes of E-cadherin, PTEN, AKT, p-AKT, BAX and BCL-2 were detected by Western blot. Results The E-cadherin protein expression was significantly decreased after E-cadherin siRNA transfection (P ＜ 0. 05 ). The IC50 of CDDP, 5-FU,paclitaxel and ADR in E-cadherin siRNA tansfected group were significantly higher than in negative control group (4. 375 ±0. 199 mg/L vs 2. 882 ±0. 166 mg/L, 6. 855 ±0. 780 mg/L vs 4. 058 ±0. 946 mg/L, 2. 530 4±0. 259mg/L vs 1. 483 4± 0. 225 mg/L, 0. 368 ± 0. 042 mg/L vs 0. 228 ± 0. 012 mg/L respectively, P ＜0. 05 ). Compared to the control group, cell proliferation was significantly increased in SGC7901 cell transfected with E-cadherin siRNA. The expressions of PTEN and BAX protein were significantly lower than the control group 72 h after trasfection ( P ＜ 0. 05), while the p-AKT and BCL-2 protin were higher than the control group ( P ＜ 0. 05 ). Conclusion E-cadherin siRNA can activate AKT pathway and regulate apoptotic balance, which may be one of mechanisms of promoting drug resistance and proliferation.关键词
E-钙黏蛋白/耐药/增殖/PTEN/凋亡Key words
E-cadherin/ drug resistance/ proliferation/ PTEN/ apoptosis分类
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陈勇,刘颖,高鸿,刘巍..E-钙黏蛋白siRNA促进胃癌细胞SGC7901耐药与增殖[J].基础医学与临床,2011,31(9):1006-1010,5.
