中国组织工程研究与临床康复2011，Vol.15Issue(28)：5131-5135,5.DOI:10.3969/j.issn.1673-8225.2011.28.001
关节软骨损伤后体外培养软骨细胞的功能变化
Functional changes in articular chondrocytes cultured in vitro after articular cartilage injury
郝鹏 1裴福兴2
作者信息
- 1. 四川省医学科学院·四川省人民医院骨科,四川省成都市,610072
- 2. 四川大学华西医院骨科,四川省成都市,610041
- 折叠
摘要
Abstract
BACKGROUND: Articular cartilage injury can influence articular chondrocyte function and induce post-traumatic osteoarthritis.OBJECTIVE: To investigate the functional changes in articular chondrocyte cultured in vitro after articular cartilage injury.METHODS: Rabbit models of articular cartilage injury were established by direct impact of low (0.9 J) and high (6.3 J) energy.Knee joint chondrocytes from normal and articular cartilage injury rabbits were isolated by enzyme digestion to observe the effects of impact on survival capacity of chondrocytes, detect the ability of chondrocytes to synthesize proteoglycan and type Ⅱ collagen, detect intracellular mRNA expression of interleukin-1? and nuclear factor-?B, and measure interleukin-1? and matrix metalloproteinase 1 levels in the culture medium.RESULTS AND CONCLUSION: After high and low energy-induced articular cartilage injury, chondrocytes showed decreased survival rate, primary cells had lower ability of adherence and growth, the intensity of toluidine blue staining and type-Ⅱ collagen immunohistochemical staining were weakened, intracellular mRNA expression of interleukin-1? and nuclear factor-?B was increased, and interleukin-1? and matrix metalloproteinase 1 levels in the culture medium were increased, and these symptoms were more obvious after high energy-induced articular cartilage i njury (P ＜ 0.05). These findings suggest that after articular cartilage injury, chondrocyte function is influenced, and the injury degree is related to traumatic intensity and the expression level of inflammatory factors.关键词
软骨细胞/软骨创伤/细胞培养/兔/白细胞介素1β/基质金属蛋白酶1/核转录因子Κb分类
基础医学引用本文复制引用
郝鹏,裴福兴..关节软骨损伤后体外培养软骨细胞的功能变化[J].中国组织工程研究与临床康复,2011,15(28):5131-5135,5.
