中国组织工程研究与临床康复2011，Vol.15Issue(28)：5201-5204,4.DOI:10.3969/j.issn.1673-8225.2011.28.018
肥大细胞在腺嘌呤致慢性肾功能衰竭模型大鼠肾组织中的浸润
Infiltration of mast cells in renal interstitium in a rat model of adenine-induced chronic renal failure
摘要
Abstract
BACKGROUND: Recent studies have shown that infiltration of mast cells (MCs) is closely correlated with the degree of tubulointerstitial fibrosis in various renal diseases. Whether MCs are involved in renal interstitial fibrosis in a rat model of adenine-induced chronic renal failure is poorly understood.OBJECTIVE: To investigate the distributing characteristics of MCs and the relationship between number of MCs in the interstitium and tubulointerstitial fibrosis.METHODS: Forty-six male Wistar rats were randomly divided into control group and model group. The model group rats were intragastrically administered adenine at a dose of 150 mg/kg per day, and the control group rats were intragastrically administered the same amount of physiological saline. Blood and urine were collected for measuring creatinine and protein.Kidney sample sections were stained with hematoxylin and eosin, Masson’s trichrome. Renal interstitial fibrosis (RIF) was assessed by light microscope. The intensity and distribution of MCs were examined by toluidine blue and immunohistochemistry using tryptase antibody.RESULTS AND CONCLUSION: Proteinuria and renal function of model rats deteriorated gradually with the elongation of intragastric administration time, and the score of RIF increased gradually. MCs were detected in the interstitium, periglomerular and perivascular areas in the model kidneys, in close proximity to areas of interstitial fibrosis, but were not found in the glomeruli.With aggravation of interstitial lesions degree, the number of MCs increased. There were significant differences in number of MCs,RIF score, urinary protein, serum creatinine and blood urea nitrogen between the model rats and control rats at different time points (P ＜ 0.01). MCs were positively correlated with interstitial fibrosis (r = 0.96, P ＜ 0.001). These findings suggest that increased infiltration of MCs is involved in renal interstitial fibrosis in a rat model of adenine-induced chronic renal failure, which provides an ideal animal model for the further research of MCs in renal tissue.关键词
肥大细胞/慢性肾功能衰竭/肾间质纤维化/类胰蛋白酶/大鼠/动物模型分类
医药卫生引用本文复制引用
邢红梅,于彩云,刘雪梅,刘洪彦..肥大细胞在腺嘌呤致慢性肾功能衰竭模型大鼠肾组织中的浸润[J].中国组织工程研究与临床康复,2011,15(28):5201-5204,4.基金项目
山东省科技攻关项目资助课题(项目编号:2010G0020222),课题名称:干细胞因子与慢性肾脏疾病的关系研究. （项目编号:2010G0020222）
