中国组织工程研究与临床康复2011，Vol.15Issue(29)：5334-5338,5.DOI:10.3969/j.issn.1673-8225.2011.29.004
冻干韧带脱细胞支架材料的生物相容性及优势
Biocompatibility and superiority of lyophilized acellular ligament scaffolds
摘要
Abstract
BACKGROUND: Acellular matrix ligament removes the cellular components within the ligament tissue and reduce the immunogenicity through a variety of acellular ways. Simultaneously, the damage to scaffold structure is mild in the process of decellularization, and it retains the mechanical properties of the extracellular matrix.OBJECTIVE: To verify the biocompatibility and superiority of rabbit patellar tendon acellular scaffold after frozen and lyophilized processing.METHODS: Patellar ligaments were treated with 1％ sodium deoxycholate for preparation of acellular ligaments with or without lyophilization. RESULTS AND CONCLUSION: No residual nuclear component was detected in all ligaments. Collagen structure was maintained.No significant differences in elastic modulus and ultimate tensile stress were found between non-lyophilized acellular scaffolds and lyophilized ones. The in vitro cytotoxicity test showed the cells grew well in all groups with or without extracts from lyophilized acellular scaffold. No significant difference was found between the control group and the experiment group. Toxicity symptoms were not obvious.Pyrogen detection experiment showed that no pyrogen was found in the lyophilized acellular scaffold extracts. Percutaneous stimulation test was negative as primary stimulation index was 0. In vivo implantation experiment showed that lyophilized acellular ligament scaffold showed the characteristics of little immunogenicity and light inflammation. Lyophilized acellular ligament scaffold treated with 1％ DCA method not only maintains the mechanical characteristics of the non-lyophilized ones, but also has good biological compatibility. Because of its preparation, disinfection, packaging and preservation was easy and convenient, the lyophilized acellular ligament scaffold will be an ideal scaffold for tissue engineering ligament.关键词
组织工程/韧带脱细胞支架/韧带/脱细胞/冻干/生物相容性分类
基础医学引用本文复制引用
王辉,陈雄生,周盛源,黄俊俊,蔡弢艺..冻干韧带脱细胞支架材料的生物相容性及优势[J].中国组织工程研究与临床康复,2011,15(29):5334-5338,5.基金项目
国家高技术研究发展计划(863 计划)项目(2008AA02Z418),课题名称:转基因骨髓间充质干细胞与脱细胞支架构建组织工程韧带的实验研究. （863 计划）
