中国组织工程研究与临床康复2011，Vol.15Issue(30)：5543-5546,4.DOI:10.3969/j.issn.1673-8225.2011.30.009
假体磨损颗粒钴铬离子影响成骨细胞增殖及RANKL、骨保护素的表达
Proliferation of mouse osteoblasts and expression of receptor activator of nuclear factor kappa B ligand and osteoprotegerin induced by cobalt and chromium ions
李俊宁 1袁晓军 1徐文华 1傅云根 1曹盛生1
作者信息
- 1. 宜春市人民医院骨二科,江西省宜春市,336000
- 折叠
摘要
Abstract
BACKGROUND: Metal-metal prosthesis can appear with corrosion or wear, and release nickel, cobalt, chromium, titanium, and some inflammatory factors locally.OBJECTIVE: To investigate the effects of cobalt (Co 2+) and chromium (Cr3+) ions on the proliferation of mouse osteoblasts and expression of receptor activator of nuclear factor kappa B ligand (RANKL) and osteoprotegerin (OPG).METHODS: Osteoblasts cultured in vitro were divided into two groups: control group with normal saline and ion group with cobalt and chromium ions.RESULTS AND CONCLUSION: The number of cells in the control group was increased remarkably at 1 -6 days after intervention, while no changes occurred in the ion group. RT -PCR results showed that the expression of RANKL and OPG was increased as compared with control group at 24 and 48 hours after coculture, especially RANKL expression (P＜ 0.05). The ratio of RANKL/OPG mRNA was also improved . Co2+ and Cr3+ ions have the remarkable inhibitory action on osteoblast proliferation and can stimulate the expression of RANKL and OPG mRNA from osteoblasts.关键词
钴离子/铬离子/成骨细胞/RANKL/骨保护素分类
基础医学引用本文复制引用
李俊宁,袁晓军,徐文华,傅云根,曹盛生..假体磨损颗粒钴铬离子影响成骨细胞增殖及RANKL、骨保护素的表达[J].中国组织工程研究与临床康复,2011,15(30):5543-5546,4.
