中国组织工程研究与临床康复2011，Vol.15Issue(33)：6187-6190,4.DOI:10.3969/j.issn.1673-8225.2011.33.026
小鼠肾小管发育过程中纤维粘连蛋白与整合素α5的表达
Fibronectin and integrin alpha 5 expression during the development of mouse renal tubule
摘要
Abstract
BACKGROUND: There are few studies describing interaction of fibronectin and integrin as influencing the development of renal tubules.OBJECTIVE: To observe the expression of fibronectin and its receptor integrin as du ring the development of mouse renal tubules.METHODS: Kunming mice at embryonic days 12, 14, 16, 18 and postnatal days 1, 3, 7, 14, 21, 28 and 40 were selected.RESULTS AND CONCLUSION: Immunohistochemical staining showed that fibronectin expression was expressed in the basement membrane of ureteric bud at embryonic 12 days and then in the basement membrane of renaltubules at any stage.Integrin as began to express at embryonic 12 days in the epithelial cells of the renal tubules. Stereological measurements showed that the surface density of fibronectin and the volume density of integ rin as increased gradually with the nephrogenesis. Westarn blot analysis showed that firbonectin and integrin as contents increased gradually with kidney development. These results demonstrate that fibronectin and interg rin as expression has certain chronological and spatial sequence during the development of mouse renal tubules, which suggest that they play an important role in the development of renal tubules.关键词
肾小管/纤维粘连蛋白/整合素α5/小鼠/发育分类
医药卫生引用本文复制引用
阎丽菁,田鹤,郭敏..小鼠肾小管发育过程中纤维粘连蛋白与整合素α5的表达[J].中国组织工程研究与临床康复,2011,15(33):6187-6190,4.基金项目
辽宁省教育厅基金项目(编号:L20100274,L20100269). （编号:L20100274,L20100269）
