中国组织工程研究与临床康复2011，Vol.15Issue(34)：6315-6318,4.DOI:10.3969/j.issn.1673-8225.2011.34.011
同种异体神经复合体修复兔周围神经缺损
Allogeneic nerve complex for repair of peripheral nerve defects in rabbits
杨小华 1韩金豹 2张沉冰 2田德虎 2韩久卉2
作者信息
- 1. 承德医学院附属医院骨外二科,河北省承德市,067000
- 2. 河北医科大学第三医院手外科,河北省石家庄市,050051
- 折叠
摘要
Abstract
BACKGROUND: Acellular nerve graft with Schwann cells is used to repair peripheral nerve defects and have a better role innerve regeneration and functional recovery with little immunogenicity.OBJECTIVE: To observe the changes of immune cells surrounding the nerve graft as well as functional recovery after acellularnerve graft with Schwann cells for repair of peripheral nerve defects.METHODS: Forty-eight adult healthy New-Zealand rabbits were randomly divided into 2 groups with 24 rabbits each group,based on different grafts. The bridging complex was composed of Schwann cells transplanted into the acellular nerve graft. Thesciatic nerve of lower left limb was cut in order to emerge 2 cm nerve defect. Only acellular nerve graft was applied in the controlgroup. The infiltrating of immune cells in the muscle tissue around allografts of the rabbit was observed after 1, 4 and 8 weeks,further, to count the amount of immune cells in each high power field. The ulcers on the feet of the rabbit and sciatic nerveregeneration were also observed at 4, 8 and 16 we eks after operation. The conducted velocity of the regenerated nerve wasexamined by the electrophysiological indices.RESULTS AND CONCLUSION: After operation, the immunological rejection was not found in the operating field. Theexperimental group was better than the control group in the healing of ulcers. There were lots of lympholeukocytes andmacrophages in the muscle tissue around the transplanted sciatic nerve after 1 week. There were obviously morelympholeukocytes and macrophages in the experimental group than the control group (P ＜ 0.05). They decreased after 4 weeksin two groups, they decreased more seriously in the experimental group, and obviously decreased after 8 weeks. There wassignificant difference between the experimental group and the control group after 1 week, but, there was not different after 4weeks and 8 weeks. After 4 weeks, the conducted velocity of the regenerated nerve was not different between two groups. But at8 and 16 weeks after operation, the experimental group had a better conducted velocity than the control group (P ＜ 0.05). Theacellular nerve allografts with Schwann cells could significantly promote nerve regeneration and functional recovery of injuredsciatic nerve. Its immunogenicity is extraordinarily low.关键词
去细胞同种异体神经/许旺细胞/周围神经缺损/神经再生/免疫排斥分类
基础医学引用本文复制引用
杨小华,韩金豹,张沉冰,田德虎,韩久卉..同种异体神经复合体修复兔周围神经缺损[J].中国组织工程研究与临床康复,2011,15(34):6315-6318,4.
