中国组织工程研究与临床康复2011，Vol.15Issue(35)：6567-6570,4.DOI:10.3969/j.issn.1673-8225.2011.35.025
静磁场作用下金属离子对成骨细胞的毒性
Static magnetic field reduces the toxic effect of metal ions on osteoblasts
摘要
Abstract
BACKGROUND: Static magnetic field can promote the proliferation, differentiation and functional activity of osteoblasts.OBJECTIVE: To investigate the toxic effect of metal ions Co2+, Cr3+ on osteoblasts under the static magnetic field.METHODS: CoCl2 powder and CrCl3 powder were dissolved in the asepsis injecting water. Mouse calvarial osteoblasts(MC3T3-E1) were cultured with Co2+ and Cr3+ ions in different magnetic field of 1, 10, 100 mT. Cells only cultured with Co2+ andCr3+ ions were taken as control group.RESULTS AND CONCLUSION: Under the static magnetic field, osteoblasts showed more mature morphological features; thetoxic effect of metal ions Co2+ and Cr3+ on osteoblasts reduced significantly; G2M (dividing phase) distribution ratio increased andG0G1 (dormancy stage) distribution ratio decreased; the A value tested by alkaline phosphatase activity in the three experimentgroups up-regulated obviously. The static magnetic field in certain intensity could antagonize the toxic effect of metal ions Co2+and Cr3+ on osteoblasts.关键词
静磁场/成骨细胞/金属离子/无菌性松动/体外实验分类
基础医学引用本文复制引用
周通华,戴闽,程细高,詹平,熊皞,邹文楠,严小青..静磁场作用下金属离子对成骨细胞的毒性[J].中国组织工程研究与临床康复,2011,15(35):6567-6570,4.基金项目
国家自然科学基金资助项目(30760255) （30760255）
南昌大学学科交叉基金(Z03722). （Z03722）
