中国组织工程研究与临床康复2011，Vol.15Issue(33)：6107-6110,4.DOI:10.3969/j.issn.1673-8225.2011.33.007
成骨细胞表面不同神经肽受体的表达
Expression of different neuropeptide receptors on osteoblast surface
张学敏 1马文辉 2时述山 3张卫平 2冯志军 2李亚非 3贾恒川 2胡兴茂 2郑晓华1
作者信息
- 1. 河北省国防建医院,河北省石家庄市,050081
- 2. 解放军白求恩国际和平医院骨科,河北省石家庄市,050082
- 3. 解放军北京军区总医院骨科,北京市,100072
- 折叠
摘要
Abstract
BACKGROUND: A large number of studies have demonstrated that neuropeptide can regulate bone metabolism. There have been five neuropeptides found to participate in the process of bone metabolism.OBJECTIVE: To observe the expression of different neuropeptides on the surface of osteoblasts of healthy adults.METHODS: Calcitonin gene-related peptide, tyrosine hydroxylase, P substance, neuropeptide Y monoclonal antibodies were used for immunohistochemical staining to observe the expression of various neuropeptide receptors on the surface of osteoblasts.In addition, semi-quantitative analysis of staining gray scale was performed through the use of computer image analysis system.RESULTS AND CONCLUSION: Neuropeptide Y, P substance and calcitonin gene-related peptide receptor expression was observed on the surface of osteoblasts of healthy adults and these factors influence cellular biological characteristics after binding with corresponding receptors. The gray value from low to high was in the following sequence, neuropeptide Y, calcitonin gene-related peptide, tyrosine hydroxylase, P substance. Results showed that neuropeptide is an important regulatory factor of biological activity of osteoblasts.关键词
神经肽/受体/成骨细胞/免疫组织化学/因子分类
基础医学引用本文复制引用
张学敏,马文辉,时述山,张卫平,冯志军,李亚非,贾恒川,胡兴茂,郑晓华..成骨细胞表面不同神经肽受体的表达[J].中国组织工程研究与临床康复,2011,15(33):6107-6110,4.
