中国组织工程研究与临床康复2011，Vol.15Issue(31)：5822-5826,5.DOI:10.3969/j.issn.1673-8225.2011.31.029
大部分肝切除模型大鼠肝再生组织中CD34蛋白及Delta-like-1蛋白的表达**☆
Expressions of CD34,Delta-like-1 protein in hepatic regeneration tissue of rats after partial hepatectomy
摘要
Abstract
BACKGROUND: When the liver affected severe damage or excalation, liver stem cells rapidly proliferated and differentiated into mature liver cells to repair liver tissue. However, liver regeneration is a complicated multiple signal molecular regulatory process and related genes and proteins that participated in regulation remain unclear. OBJECTIVE: To investigate the expressions of CD34 and Delta-like-1 protein in rat liver regenerated tissues after partial hepatectomy. METHODS: Rat hepatic regeneration models were established after hepatectomy. Expressions of ATPase, proliferating cell nuclear antigen (PCNA), CD34 and Delta-like-1 protein were detected by hematoxylin-eosin staining, enzyme-histochemistry and immunohistochemistry method at various time points. RESULTS AND CONCLUSION: At the early stage of liver regeneration, the expression of ATPase was down-regulated, and the expressions of CD34 and Delta-like-1 protein were up-regulated. At the middle stage, the expression of ATPase was increased to the normal level, and the expressions of CD34 and Delta-like-1 protein were down-regulated. The expression of PCNA protein was continuously up-regulated. These suggest that the expressions of ATPase, CD34 and Delta-like-1 protein were different at different stages of hepatic regeneration, which means that different signal molecules were cooperated during the hepatic regeneration, and Notch-Delta signal pathway takes part in the pathogenesy.关键词
肝再生/CD34/Delta-like-1/ATP酶/增殖细胞核抗原分类
基础医学引用本文复制引用
杨志云,侯艺鑫,王宪波..大部分肝切除模型大鼠肝再生组织中CD34蛋白及Delta-like-1蛋白的表达**☆[J].中国组织工程研究与临床康复,2011,15(31):5822-5826,5.基金项目
国家自然科学基金项目(30873423) （30873423）
北京市中医管理局青年科学研究资助项目(QN2010-7).the National Natural Science Foundation of China,No.30873423* （QN2010-7）
the Youth Science Research Program of Administration of Traditional Chinese Medicine of Beijing City,No.QN2010-7 （）
