中国组织工程研究与临床康复2011，Vol.15Issue(34)：6271-6275,5.DOI:10.3969/j.issn.1673-8225.2011.34.001
血管内皮生长因子缓释系统复合成骨诱导的骨髓间质干细胞修复骨缺损
Vascular endothelial growth factor delivery system combined with bone marrow mesenchymal stem cell induced by osteogenesis for repair of bone defects
徐晓峰 1王明伟 2潘鑫 3刘小平 2张志坚4
作者信息
- 1. 江苏大学附属医院骨科,江苏省镇江市,212013
- 2. 江苏大学临床医学院,江苏省镇江市,212013
- 3. 镇江市急救中心,江苏省镇江市,212013
- 4. 江苏大学基础医学与医学技术学院,江苏省镇江市212013
- 折叠
摘要
Abstract
BACKGROUND: Present studies have reported that the delivery system of growth factors applied in orthopedics is focused onthe repair of cartilage. However, the report of using composite tissue engineering bone to repair bone defect is scarce.OBJECTIVE: To construct a composite scaffold of nano-hydroxyapatite/collagen (nHAC) modified by bone marrow mesenchymalstem cells (BMSCs) with osteogenic induction and controlled releasing vascular endothelial growth factor (VEGF) delivery systemconstituted of VEGF, heparin (HP) and fibrin (FB), and investigate the effect of repair of bone defect.METHODS: Composite scaffold delivery system was constructed with different combinations of VEGF, HP and FB. Thenosteogenic induced BMSCs were seeded onto the nHAC scaffolds loaded with the optimal controlled releasing VEGF deliverysystem. Animal models of femoral bone defect were established. A total of 36 SD rats were randomly divided into 3 groups:VEGF/HP/FB/nHAC/BMSCs group, VEGF/HP/FB/nHAC group and control group, all groups administratered with internal fixation.The releasing capacity of VEGF was estimated by ELISA. X ray and alkaline phosphatase (ALP) activity observation wereperformed at 1, 4, 12, 24 weeks after implantati on to evaluate the repair of bone defects in each group.RESULTS AND CONCLUSION: nHAC modified by controlled releasing drug delivery system constituted of VEGF, HP and FBstill released VEGF at a high concentration at 30 days in vitro , and the releasing curve was flat. The composite scaffold in animalmodel was completely degraded at 24 weeks. Bone defects were repaired well and ALP activity in bone defective parts wassignificantly higher in VEGF/HP/FB/nHAC/BMSCs group than that in VEGF/HP/FB/nHAC and control groups. nHAC modified bycontrolled releasing drug delivery system constituted of VEGF, HP and FB has a better releasing characteristic, and thecomposite scaffold of VEGF/HP/FB/nHAC/MSCs can promote the speed and quality of bone repair in bone defect rat models.关键词
血管内皮生长因子/缓释系统/骨髓间充质干细胞/纳米晶胶原基骨/复合组织工程骨分类
基础医学引用本文复制引用
徐晓峰,王明伟,潘鑫,刘小平,张志坚..血管内皮生长因子缓释系统复合成骨诱导的骨髓间质干细胞修复骨缺损[J].中国组织工程研究与临床康复,2011,15(34):6271-6275,5.
