中国组织工程研究与临床康复2011，Vol.15Issue(34)：6276-6280,5.DOI:10.3969/j.issn.1673-8225.2011.34.002
重组人骨形态发生蛋白2-肝素-人工骨复合材料的骨诱导活性
Bioactivities of recombinant human bone morphogenetic protein 2-heparin artificial bone composite materials
摘要
Abstract
BACKGROUND: Recombinant human bone morphogenetic protein 2 (rhBMP-2) has been applied to bone regeneration andrepairing, but its ability to induce bone formation is limited because of its short half-life in vivo.OBJECTIVE: To prepare rhBMP-2-heparin composite materials and to evaluate rhBMP-2 sustained delivery and osteoinductiveeffects.METHODS: The effect of heparin on protecting rhBMP-2 from enzyme digestion was assayed by high-performance liquidchromatography. The materials were combined with rhBMP-2-heparin to produce rhBMP-2-heparin artificial bone compositematerials, detecting the rhBMP-2 release effect by ELISA. Alizarin red staining was used to detect osteogenic ability. The ability toinduce ectopic bone was detected through an in vivo mouse experiment.RESULTS AND CONCLUSION: It is successful to prepare rhBMP-2 artificial composite materials with good sustained releaseeffect of rhBMP-2, which can effectively induce osteocalcin and ectopic bone formation.关键词
重组人骨形态发生蛋白2/肝素/人工骨复合材料/缓释/骨形成/异位骨分类
基础医学引用本文复制引用
周智优,何水连,孙莉,王丁丁,汪炬..重组人骨形态发生蛋白2-肝素-人工骨复合材料的骨诱导活性[J].中国组织工程研究与临床康复,2011,15(34):6276-6280,5.基金项目
省科技攻关项目(2009B030801237) （2009B030801237）
广东省教育部科技部企业科技特派员行动计划项目(2009B090600134). （2009B090600134）
