中国病理生理杂志2011，Vol.27Issue(9)：1741-1745,5.DOI:10.3969/j.issn.1000-4718.2011.09.015
低氧-复氧对人鼻咽癌细胞增殖、细胞周期、黏附和侵袭能力的影响
Effects of hypoxia-reoxygenation on proliferation, cell cycle,adhesion and invasion of human nasopharyngeal carcinoma cells
摘要
Abstract
AIM: To investigate the effects of hypoxia - reoxygenation on the proliferation, cell cycle, adhesion and invasion of human nasopharyngeal carcinoma CNE - 2Z cells.METHODS: Hypoxic culture condition for CNE -2Z cells was established by equilibration of cell monolayer growing in an air - tight chamber with O2 - deficient gas mixture ( 95％ N2, 5％CO2 and ＜0.1％ O2 ).MTT assay was used for observing the cell proliferation.The cell cycle was analyzed by flow cytometry.The adhesion ability of the cells was tested by homotypic adhesion assay and cell - matrix adhesion assay.The invasion ability was assessed by Transwell method.RESULTS: ( 1 ) Growth curve showed a temporary and reversible inhibition of proliferation in CNE - 2Z cells under hypoxic culture condition for 24 h.After reoxygenation for 5 days, the cell proliferation recovered and showed no significant difference compared with that under normal culture condition (P＞0.05).(2) Hypoxia for 24 h induced G1 arrest in CNE - 2Z cells.After reoxygenation for 24 h, G1 arrest was released and the cells entered into normal cell cycle progress.( 3 ) Compared with those of normoxic cells, the homotypic adhesion and invasion of 24 - hour hypoxic cells were decrease ( P ＜ 0.01 ) while the cell - matrix adhesion showed no significant difference ( P ＞0.05 ).In contrast, the cell - matrix adhesion displayed a time - dependent increase after reoxygenation.The invasion ability also enhanced after reoxygenation.CONCLUSION: Hypoxia induces adaptive responses of nasopharyngeal carcinoma cells, which may endow nasopharyngeal carcinoma with greater proliferative and metastatic potentials.关键词
低氧/鼻咽肿瘤/细胞增殖/细胞周期/肿瘤侵袭Key words
Hypoxia/ Nasopharyngeal neoplasms/ Cell proliferation/ Cell cycle/ Neoplasm invasiveness分类
医药卫生引用本文复制引用
杨海峰,朱林燕,郑广娟,陈丽新,王立伟,林熙,唐慰萍..低氧-复氧对人鼻咽癌细胞增殖、细胞周期、黏附和侵袭能力的影响[J].中国病理生理杂志,2011,27(9):1741-1745,5.基金项目
国家自然科学基金资助项目(No.31070997) （No.31070997）
广东省卫生厅医学科技资助项目(No.A2010314) （No.A2010314）
广州中医药大学中医药科研创新基金资助项目(No.10CX49) （No.10CX49）
