中国病理生理杂志2011，Vol.27Issue(9)：1796-1801,6.DOI:10.3969/j.issn.1000-4718.2011.09.026
ERK通路和三七总皂苷干预在低氧高二氧化碳性肺动脉高压中的作用
Roles of ERK pathway and Panax notoginoside treatment in hypoxic hypercapnia pulmonary hypertension in rats
摘要
Abstract
AIM: To investigate the roles of Panax notoginoside ( PNS ) and ERK1/2 signaling pathway in the pathological process of chronic hypoxic hypercapnia pulmonary hypertension in rats.METHODS: The animal model of chronic hypoxic hypercapnia pulmonary hypertension was set up in 72 male Sprague - Dawley rats and the animals were randomly divided into 6 groups: normal ( N ) group, hypoxic hypercapnia for 3 - day ( H3d ) group, hypoxic hypercapnia for 1 - week ( H1w ) group, hypoxic hypercapnia for 2 - week ( H2w ) group, hypoxic hypercapnia for 4 - week ( H4w ) group and PNS treatment ( Hp ) group.The rats in Hp group were injected with PNS ( 50 mg · kg-1 · d-1 , ip ) before placing the animals into the hypoxic hypercapnia chamber.The rats in other groups were injected with normal saline ( 2 mL/kg, ip ).The morphological changes of the pulmonary artery were observed under microscope with HE staining.Western blotting was used to detect the protein expression of p - ERK.The protein levels of p - ERK in the lung tissues and pulmonary blood vessels were determined by immunohistochemistry.RESULTS: The ratios of WA/TA in H1w , H2w , H4w and Hp groups were higher than that in N group ( P ＜ 0.05 ).The ratio of WA/TA in Hp group was obviously lower than that in H4w group ( P ＜ 0.05 ).The protein expression of p - ERK was barely positive in N group, but was up - regulated in the pulmonary tissues in all hypoxic rats.Compared with N group, the protein level of p - ERK was markedly up - regulated in H3d group, reached its peak in H2w group, and tended to decline in H4w group ( P ＜0.05 ).In pulmonary arterial tunica intima and tunica media, p - ERK protein was dramatically expressed in all hypoxic rats compared with the control animals ( P ＜ 0.05 ).In the lung tissues, the protein level of p - ERK in H group was lower than that in H4w group ( P ＜0.05 ).In pulmonary arterial tunica intima and tunica media, the protein level of p - ERK in Hp group was lower by 84.86％ than that in H4w group ( P ＜0.05 ).CONCLUSION: ERK1/2 as a signal transducer may play an important role in the development of hypoxia and hypercapnia induced pulmonary hypertension.PNS inhibits the expression of ERK1/2, thus attenuating the development of pulmonary hypertension and improving pulmonary vascular remodeling.关键词
三七总皂苷/低氧/高碳酸血症/肺动脉/ERK通路Key words
Panax notogino side/ Hypoxia/ Hypercapnia/ Pulmonary artery/ ERK pathway分类
医药卫生引用本文复制引用
朱阿楠,王园园,王淑君,金可可,汪洋,王万铁..ERK通路和三七总皂苷干预在低氧高二氧化碳性肺动脉高压中的作用[J].中国病理生理杂志,2011,27(9):1796-1801,6.基金项目
浙江省科技攻关资助项目(No.2006C33073) （No.2006C33073）
