中国病理生理杂志2011，Vol.27Issue(9)：1848-1851,4.DOI:10.3969/j.issn.1000-4718.2011.09.038
小胶质细胞体外吞噬模型的建立及相关炎症因子的研究
An in vitro phagocytosis model of microglia and factors related to inflammation
摘要
Abstract
AIM: To establish a phagocytosis model of microglia mediated by apoptotic signal phosphatidylserine ( PS ) and to explore the expression of inflammatory cytokines in the phagocytic process of microglia.METHODS: The apoptosis model of red blood cells ( RBC ) was established by incubating RBC with oxidized PS( PSox ) liposomes after pretreatment of RBC with N - ethylmaleimide ( NEM ).The phagocytic rate of microglia to RBC integrated with oxidation of PS signal was measured.Real - time PCR was used to detect the expression of inflammatory cytokines such as interleukin -1 beta ( IL - 1 β ) and tumor necrosis factor alpha ( TNF - α ) in microglia.RESULTS: RBC incubated with PSox liposomes significantly enhanced the phagocytic rate of microglia.Inflammatory cytokines such as IL - 1 β and TNF - α were apparently decreased at the transcription level when microglia exerted phagocytosis on RBC.CONCLUSION: The in vitro phagocytosis model of microglia was established by integrating PSox with RBC directly.The microglia decreases the expression of inflammatory cytokines at the transcription level in the phagocytic process when RBC were integrated with oxidation of PS signal.关键词
小神经胶质细胞/磷脂酰丝氨酸类/吞噬作用/细胞因子类Key words
Microglia/ Phosphatidylserines/ Phagocytosis/ Cytokines分类
医药卫生引用本文复制引用
聂盼,刘勇,杨学森,肖明春,刘堰..小胶质细胞体外吞噬模型的建立及相关炎症因子的研究[J].中国病理生理杂志,2011,27(9):1848-1851,4.基金项目
国家自然科学基金资助项目(No.30700931 （）
No.30970672) （）
