中国比较医学杂志2011，Vol.21Issue(8)：1-4,封2,5.DOI:10.3969/j.issn.1671-7856.2011.08.001
呼肠孤病毒Ⅲ型免疫荧光检测方法的建立及初步应用
Establishment and Initial Application of an Immunofluorescence Assay in Detecting Mammalian Orthoreovirus 3
摘要
Abstract
Objective To establish an immunofluorescence assay (IFA) method of detecting the mammalian orthoreovirus 3 ( Reo3 ) in animal-derived biological materials and biological products for human use. Methods Reo3sensitive cells were screened. The virus TCID50was titrated and antigen slides were prepared according to GB IFA. The best working concentration of IgG-FITC was titrated and the specificity, sensitivity and stability were tested. Results BSC-1 cells were selected as Japanese encephalitis virus (JEV)-sensitive cells. The TCID50 of the virus was 10-5.8· mL-1. The best working concentration of the IgG-FITC was 1: 100. There was no cross-reactivity with ectromelia virus and mouse hepatitis virus. The tests of sensitivity and stability showed that the detection sensitivity was 1: 1280 at different time points. The lowest detected virus titer was 10 -4. 1. mL -1. Conclusions The established IFA method is good in sensitivity,specificity and stability, and can be used in detecting the Reo3 in animal-derived biological materials and biological products for human use.关键词
呼肠孤病毒Ⅲ型/人用动物源性生物材料及生物制品/免疫荧光试验Key words
Mammalian orthoreovirus 3/ Biological materials, human use/ Biological products, human use/Immunofluorescence assay分类
医药卫生引用本文复制引用
王吉,卫礼,岳秉飞,贺争鸣..呼肠孤病毒Ⅲ型免疫荧光检测方法的建立及初步应用[J].中国比较医学杂志,2011,21(8):1-4,封2,5.基金项目
"国家科技支撑计划":疫苗类生物制品安全性评价技术研究(2008BAI54B01). （2008BAI54B01）
