中国比较医学杂志2011，Vol.21Issue(8)：5-9,封2,6.DOI:10.3969/j.issn.1671-7856.2011.08.002
雪貂感染流感病毒H3N2动物模型的建立
Establishment of a Ferret Model of H3N2 Influenza Virus Infection
摘要
Abstract
Objective To establish a ferret model of H3N2 influenza virus infection. Methods Six ferrets were divided into two groups for three each. after anesthetized by ketamine, the ferrets were inoculated intranasally with 500 μL of 106 and 107TCID50 H3N2 A/Brisbane/10/07 influenza virus, respectively. Clinical signs were monitored daily, and body temperature was recorded once every day postinfection. Nasal turbinate biopsies were taken every day from day 0 to postinfection day 5 (d 0 to d. p. i. 5) for real time PCR analysis, the ferrets were euthanized and the lung, liver, slpeen,small intestine and brain tissues were harvested at d. p. i. 5. The lungs were further examined by histopathology.Results For each virus titer tested, transient elevation of body temperature, weight loss, nasal rattling and sneezing were observed in the infected ferrets, but no ferret died. Virus was detected positively in the nasal turbinates and small intestine from all the infected ferrets. The ferrets inoculated with H3N2 virus showed mild interstitial pneumonia in the lung.Conclusions The ferrets were successfully infected with H3N2 influenza virus proved by clinical signs, virus detection,and histological examination. Furthermore, 106 TCID50 titer is a more appropriate dose for establishment of a ferret model of flu H3N2 virus infection.关键词
A/Brisbane/10/07/H3N2/雪貂/模型,动物Key words
Influenza virus, H3N2 strain/ A/Brisbane/10/07/ Ferret/ Model, animal分类
医药卫生引用本文复制引用
鲍琳琳,占玲俊,邓巍,许黎黎,朱华,吕琦,李枫棣,秦川..雪貂感染流感病毒H3N2动物模型的建立[J].中国比较医学杂志,2011,21(8):5-9,封2,6.基金项目
科技重大专项-艾滋病和病毒性肝炎等重大传染病防治:2009ZX10004-402 （）
卫生公益性行业科研专项项目:200802036 （）
中央级公益性科研院所基本科研业务费:DWS200810. （）
