中国神经再生研究（英文版）2011，Vol.6Issue(22)：1709-1714,6.DOI:10.3969/j.issn.1673-5374.2011.22.005
Hypothermic intervention for 3 hours inhibits apoptosis in neonatal rats with hypoxic-ischemic brain damage
Hypothermic intervention for 3 hours inhibits apoptosis in neonatal rats with hypoxic-ischemic brain damage
Yale Guo 1Zhankui Li 2Ruilin Li 1Baoshan Su 3Shaoping Huang 1Jianping Zhou1
作者信息
- 1. Department of Pediatrics, Second Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China
- 2. Department of Neonatology, Maternal and Child Health Hospital of Shaanxi Province, Xi'an 710004, Shaanxi Province, China
- 3. Department of Pathology, Second Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China
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摘要
Abstract
A neonatal rat model of hypoxic-ischemic brain damage was designed and implemented in this study.Rats were subjected to hypothermia at 31°C immediately follow ing hypoxia-ischemia for either 3, 6 or 15 hours.TdT-mediated dUTP nick end labeling demonstrated that the number of apoptotic cells was reduced in the rat cerebral cortex, hippocampus and periventricular white matter following hypothermia.Immunohistochemistry revealed that Bcl-2 and p16 expression were decreased.Inhibition of apoptosis was greatest with the 3 hour hypothermic treatment, followed by hypothermia for 6 hours.In contrast, hypothermia for 15 hours led to a decrease in neuronal number in the cerebral cortex.The results demonstrate that hypothermic intervention at 31°C protects brain tissue against hypoxic-ischemic brain damage by inhibiting apoptosis, and that the optimal length of treatment is 3 hours.关键词
hypothermia/hypoxia/ischemia/neural protection/neural regenerationKey words
hypothermia/hypoxia/ischemia/neural protection/neural regeneration引用本文复制引用
Yale Guo,Zhankui Li,Ruilin Li,Baoshan Su,Shaoping Huang,Jianping Zhou..Hypothermic intervention for 3 hours inhibits apoptosis in neonatal rats with hypoxic-ischemic brain damage[J].中国神经再生研究（英文版）,2011,6(22):1709-1714,6.
