肿瘤防治研究2011，Vol.38Issue(8)：857-860,4.DOI:10.3971/j.issn.1000-8578.2011.08.002
氧化应激对大肠癌细胞迁移、血管内皮生长因子表达及细胞间通信的影响
Effect of Oxidative Stress Cell Migration,Vascular Epithelial Growth Factor Expression and Gap Junction Intercellular Communication on Colon Cancer
摘要
Abstract
Objective To investigate the effects of oxidative stress on colon cancer cell migration, vascular epithelial growth factor expression and gap junction intercellular communication. Methods We stimulated HCT-8 human colon cancer cell with different concentration of hydrogen peroxide, through which mimicking the oxygen stress environment in the colon lumen was devedoped. We observed the colon cancer cell migration using Transwell Chamber assay, the expression of VEGF gene using immunocytochemistry, the gap junction intercellular communication using the scrape loading/dye transfer technique,and furthermore the expression of intercellular communication protein Connexin 43 using Western blot.Results The oxygen stress environment induced by hydrogen peroxide at concentration ≤ 10-5 mol/L shightly promoted and concentration of 10-5 mol/L significantly promoted the colon cancer migration (t =2.247, P＜0.05), up-regulated the expression of VEGF gene (Z= 3.938, P= 0.000) and inhibited the intercellular communication (Z= 3.938, P = 0.000). Conclusion Oxidative stress mimicked by hydrogen peroxide might be involved in colorectal tumor development through promotion of the colon cancer cell migration, up-regulation of VEGF gene and inhibition of the intercellular communication.关键词
氧化应激/大肠癌细胞/细胞迁移/血管内皮生长因子/细胞通信Key words
Hydrogen peroxide/Colon cancer cell/Cell migration/ Vascular epithelial growth factor/ Gap junction intercellular communication分类
医药卫生引用本文复制引用
刘培根,马利林,朱建伟..氧化应激对大肠癌细胞迁移、血管内皮生长因子表达及细胞间通信的影响[J].肿瘤防治研究,2011,38(8):857-860,4.基金项目
国家自然科学基金资助项目(30771126) （30771126）
教育部留学回国人员基金资助项目(890) （890）
江苏省自然科学基金资助项目(BK2006058) （BK2006058）
