肿瘤防治研究2011，Vol.38Issue(8)：861-865,5.DOI:10.3971/j.issn.1000-8578.2011.08.003
鞘氨醇激酶-1激活ERK通路介导人结肠癌细胞株LoVo侵袭与迁移的实验
Sphingosine Kinase-1 Regulates LoVo Cell Invasion and Migration via Activation of ERKl/2 Pathway
摘要
Abstract
Objective To investigate the effect of Sphk1 on colon cancer cell invasion and migration. Methods Human colon cancer LoVo cells were divided into three group: LoVo cells were treated using 100 nM Phorbol 12-myristate 13-acetate (PMA) as the Sphk1 activation group, 5(0 μM N, N-dimethyl-Derythro-sphingosine (DMS) as suppression group, and 0. 9％NaC1 as control group. Cell invasiveness and migration were detected by Transwell boyden chamber model. Sphk1, ERK1/2, p-ERK1/2 protein expressions were detected by Western blot, MMP-2, MMP-9 and uPA protein levels in the culture medium were detected by enzyme-linked immunosorbent assay (ELISA), and MMP-2, MMP-9 and uPA mRNA expressions in LoVo cells were detected by semi-quantitative reverse transcription-polymerase chain reaction. Results The Sphk1 activator induced the expression of Sphk1 and obviously enhanced LoVo cell invasion and migration capacity, accompanied with the up-regulating of ERK1/2, p-ERK1/2 protein expressions: moreover the protein in culture medium and the mRNA in cells levels of MMP-2, MMP-9 and uPA were elevated. On the contrary, the inhibitor obviously suppressed the protein expression of Sphk1 and cell invaseness and migration, associated with the suppressing of ERK1/2, p-ERK1/2 protein expressions; furthermore, the protein and the mRNA levels of MMP-2, MMP-9 and uPA were down-regulated. Conclusion Sphk1 was able to promote the invasion and migration of LoVo cells, through the activation of ERK1/2 signaling pathway, MMP-2, MMP-9 and uPA mRNA expression were up-regulated.关键词
鞘氨醇激酶-1/人结肠癌细胞/侵袭/迁移Key words
Sphingosine kinase 1/ Human colon cancer cells/ Invasion/ Migration分类
医药卫生引用本文复制引用
钟月圆,刘诗权,黄杰安,覃蒙斌,金卉..鞘氨醇激酶-1激活ERK通路介导人结肠癌细胞株LoVo侵袭与迁移的实验[J].肿瘤防治研究,2011,38(8):861-865,5.基金项目
国家自然科学基金资助项目(30760275) （30760275）
广西科学基金资助项目(0832008) （0832008）
