中国组织工程研究与临床康复2011，Vol.15Issue(37)：6947-6950,4.DOI:10.3969/j.issn.1673-8225.2011.37.025
不同葡萄糖培养条件对L6骨骼肌细胞中活性氧的生成作用
Reactive oxygen species formation induced by different concentrations of glucose in L6 cells
摘要
Abstract
BACKGROUND: Reactive oxygen species (ROS) formation induced by treatment with different culture conditions of glucose inL6 cells is the basis for studying the pathogenesis of type 2 diabetes mellitus.OBJECTIVE: To investigate the generation of intracellular ROS induced by treatment with different concentrations of glucoseand different culture time periods.METHODS: L6 skeletal muscle cells cultured in vitro and L6 myotube cells differentiated were treated with DMEM containing 3,5.5 and 25 mmol/L glucose and ROS formation was determined.RESULTS AND CONCLUSION: With prolonged culture time, intracellular ROS formation increased. ROS formation wassignificantly accumulated in cells treated with 25 mmol/L glucose than in cells treated with 5.5 mmol/L glucose by day 4. Glucoseat a concentration of 3 and 5.5 mmol/L did not produce significant effects on ROS formation. ROS formation in L6 myotubescultured by high concentration of glucose was more obvious than that in L6 mysoblast. Results showed that glucoseconcentration, culture time and the differentiation degree of in vitro cultured cells are the factors influencing ROS formation and invitro culture of cells by glucose can be used to mimic the chronic damage effect of hyperglycemia in vivo.关键词
高糖/低糖/活性氧/骨骼肌细胞/肌管细胞/损伤分类
医药卫生引用本文复制引用
庞婧,代杨,宫环,张恩毅,张铁梅..不同葡萄糖培养条件对L6骨骼肌细胞中活性氧的生成作用[J].中国组织工程研究与临床康复,2011,15(37):6947-6950,4.基金项目
国家自然科学基金面上项目(30971395),课题名称:多因素综合作用下的血糖变化趋势判定研究. （30971395）
