中国药理学通报2011，Vol.27Issue(10)：1378-1382,5.DOI:10.3969/j.issn.1001-1978.2011.10.011
细胞因子信号传导抑制蛋白1对肿瘤坏死因子-α诱导的肾小管细胞凋亡的影响
Influence of SOCS-1 on TNF-α-induced renal tubular epithelial cells apoptosis
摘要
Abstract
Aim To investigate the effects of suppressor of cytokine signaling-l ( SOCS-1 ) on tumor necrosis factor alpha ( TNF-α ) induced apoptotic cell death and activation of JAK/STAT in cultured human renal tubular epithelial cells ( HKC ). Methods Stable transfections of HKC with pCR3. I vector and pCR3. 11SOCS-I were performed with Lipofectamine 2000, and cells were selected with geneticin. Cells were stimulated with TNF-a( 20 μg · L-1 ). The protein expressions of SOCS-I. Bax, Bcl-2. Cleaved caspase-3,Caspase-3 Janus kinase 2/ signal transducer and activator of transcription I ( JAK2, STATI ), p-JAK2 and p-STATI were observed by Western blot. Apoptosis rate of HKC induced by TNF-a was analyzed by flow cytormetry ( FCM ). DeadEndrM Colorimetric TUNEL System detection kit were used to observe the number of apoptotic cells. Results Compared with control group, the number of cell apoptosis significantly increased, meanwhile, the expression levels of Cleaved caspase-3 protein and Bax/Bcl-2 were significantly increased in HKC with TNF-a stimulation. Overexpression of SOCS-1 inhibited TNF-a-induced cell apoptosis, activation of JAK2 and STATl,and expression of Cleaved caspase-3 protein, as well as the ratio of Bax/Bcl-2. Conclusion Overexpression of SOCS-I may inhibit TNF-α-induced renal tubular epithelial apoptosis partly through blocking activation of JAK/STAT.关键词
细胞因子信号传导抑制蛋白1/肿瘤坏死因子-α/肾小管上皮细胞/JAK2/STAT1/凋亡/基因转染Key words
suppressor of cytokine signaling-l / tumor necrosis factor alpha/ renal tubular epithelial cells/JAK2/STATI / apoptosis / genetransfection分类
医药卫生引用本文复制引用
杜春阳,史永红,崔立文,任韫卓,王月华,赵松,段惠军..细胞因子信号传导抑制蛋白1对肿瘤坏死因子-α诱导的肾小管细胞凋亡的影响[J].中国药理学通报,2011,27(10):1378-1382,5.基金项目
国家自然科学基金资助项目(No 30971119) （No 30971119）
高等学校博士学科点专项科研基金资助项目(No 200800890001) （No 200800890001）
河北省自然科学基金资助项目(No C20090001083) （No C20090001083）
