中国药理学通报2011，Vol.27Issue(10)：1413-1417,5.DOI:10.3969/j.issn.1001-1978.2011.10.019
辣椒素受体参与骶髓后联合核神经元突触传递
Involvement of capsaicin receptors in synaptic transmission in sacral dorsal commissural nucleus neurons
摘要
Abstract
Aim To investigate the role of capsaicin receptors in synaptic transmission in sacral dorsal commissural nucleus ( SDCN ) neurons of rat spinal cord.Methods Whole-cell voltage-clamp recordings on spinal cord slice were used to investigate glutamate-mediated excitatory postsynaptic currents ( EPSCs ) and γ-aminobutyric acid ( GABA )-mediated inhibitory postsynaptic currents ( IPSCs ); capsaicin effect on action potentials discharge was also accessed. Results Activation of capsaicin receptors by a specific agonist , capsaicin ( I μmol · L-1 ). significantly enhanced both frequency and amplitude of spontaneous EPSCs ( sEP-SCs ) ( P ＜ 0. 05 , n = 17 ). In the presence of tedrodotoxin ( TTX ), capsaicin increased miniature EPSCs mEPSCs ) frequency ( P ＜ 0. 01 , n = 13 ) but not amplitude distribution ( P ＞ 0. 05 , n = 13 ), indicating a presynaptic action. Capsaicin also significantly accelerated action potential discharge ( P ＜ 0. 01， n = 19 ).Capsaicin action was abolished by pretreatment of a specific antagonist, capsazepine. Capsaicin also enhanced spontaneous IPSCs ( sIPSCs ) frequency( P ＜0. 05, n = 20 ), but had no effect on either frequency or amplitude of miniature IPSCs ( mIPSCs ) ( P ＞0. 05 , n = 9 ). Conclusion Capsaicin receptors are exclusively expressed at presynaptic terminals of excitatory glutamatergic terminals to SDCN neurons; activation of capsaicin receptors modulates excitatory and inhibitory synaptic transmission in SDCN which may contribute to nociceptive information transmission and modulation at spinal cord level.关键词
骶髓后联合核/谷氨酸/β-氨基丁酸/辣椒素受体/全细胞记录/脊髓/大鼠Key words
sacral dorsal commissural nucleus / glutamate/ GABA/ capsaicin receptors/ whole-cell recordings/ spinal cord/ rat分类
医药卫生引用本文复制引用
马红雨,任曲,魏利召,罗丹,安映红,杨鲲..辣椒素受体参与骶髓后联合核神经元突触传递[J].中国药理学通报,2011,27(10):1413-1417,5.基金项目
国家自然科学基金资助课题(No 30000052) （No 30000052）
