中国药理学通报2011，Vol.27Issue(10)：1451-1457,7.DOI:10.3969/j.issn.1001-1978.2011.10.028
淫羊藿次苷Ⅱ通过激活雌激素信号通路促进骨髓间充质干细胞的成骨性分化
Promotion of the osteogenic differentiation of rat bone marrow stromal cells via estrogen signal pathway by icariside Ⅱ
摘要
Abstract
Aim To investigate the effects on the estrogen signal pathway in the osteogenic differentiation process of rat bone marrow stromal cells( rBMSCs ) by icariside Ⅱ.Methods rBMSCs were cultured by adherence screening method, icariside Ⅱ was supplemented into the culture at 10 -5 mol · L-1.The expression of estrogen signal pathway ' s factors ( ERα, PR,PS-2 ) and osteogenic differentiation markers including alkaline phosphatase ( ALP ) activity, osteocalcin and calcium deposition were compared among the icariside Ⅱ -supplemented group, ICI group, icariside Ⅱ + ICIgroup, estrogen group, estrogen + ICI group and the control.Total RNA was isolated and the gene expression of ERα, PR. PS-2, Osterix and Runx-2 were investigated by Real Time RT-PCR.Total protein was also isolated and the secretion of ERα, PR, PS-2 and collagen Ⅰ were examined by Western blot.Results Icariside Ⅱ significantly improved ALP activity, calcium deposition and the secretion of collagen Ⅰ and osteocalcin.Besides, the mRNA level of factors related to the osteogenic differentiation including Osterix and Runx-2 also enhanced.But all of these effects were inhibited by ICI 182.780 which could specifically inhibit the estrogen signal pathway.Conclusions Icariside Ⅱ enhances the osteogenic differentiation of rBMSCs significantly , but the osteogenic differentiation markers decrease accompanied with the estrogen signal pathway inhibited by ICI 182.780, suggesting that icariside Ⅱstimulates the osteogenic differentiation via activating the estrogen signal pathway.关键词
淫羊藿次苷Ⅱ/骨髓间充质干细胞/成骨性分化/I型胶原/雌激素信号通路/雌激素受体α/ICI 182.780Key words
icariside Ⅱ / bone marrow stromal cells/ osteogenic differentiation/ collagen Ⅰ/ estrogen signal pathway/ ERα / ICI 182.780分类
医药卫生引用本文复制引用
翟远坤,陈克明,葛宝丰,马慧萍,明磊国,程国政..淫羊藿次苷Ⅱ通过激活雌激素信号通路促进骨髓间充质干细胞的成骨性分化[J].中国药理学通报,2011,27(10):1451-1457,7.基金项目
甘肃省科技重大专项计划资助项目(No 092NKDA025) （No 092NKDA025）
